International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (75) Last page : (81)
Article doi: http://dx.doi.org/10.18782/2320-7051.2226
Studies on the Effect of Various Sterilization Procedure for in vitro Seed Germination and Successful Micropropagation of Cucumis sativus
Firoz Alam M1*, Ekhlas Uddin M1, Ruhul Amin1, Abdur. M. Razzak2, M.A. Manik3, and M.M. Khatun4
1Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
2Pohang University of Science and Technology, Pohang 790–784, South Korea
3Department of Biotechnology and Genetic Engineering, Khulna University, Khulna, Bangladesh
4National Institute of Biotechnology, Ganakbari, Savar, Dhaka-1349, Bangladesh
*Corresponding Author E-mail: firozbt21@gmail.com
Received: 18.02.2016 | Revised: 25.02.2016 | Accepted: 27.02.2016
Abstract
Cucumber (Cucumis sativus L.) is one of the most economically important cucurbit which serve as a good source of nutrition. It plays a significant role in pharmaceutical sectors and also used as an Eco-Friendly Pesticides. An efficient in vitro protocol was developed for micro-propagation of C. sativus to meet the demand in tissue culture lab.The most effective treatment for sterilization of explant was evaluated with four different sterilizing agents i.e: Mercuric (II) chloride (HgCl2), Sodium hypochlorite (NaOCl), Hydrogen peroxide (H2O2) and Bavistin. The percentage of contamination, survival rate, shoot length and growth pattern of cultures were studied. Among all experiment, 0.1% Mercuric (II) chloride at 4 min. was showed most effective treatment with highest rate of contamination free culture where the survival rate was 84.44%. Whereas, sterilization with Hydrogen peroxide, Sodium hypochlorite, Bavistin did not get any satisfactory result.
Key words: Cucumber,Sterilization, in vitro, Explants, growth regulators.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Firoz, M.A., Uddin M.E., Amin, R., Razzak, M.A., Manik, M.A. and Khatun, M.M., Studies on the Effect of Various Sterilization Procedure for in vitro Seed Germination and Successful Micropropagation of Cucumis sativus, Int. J. Pure App. Biosci. 4(1): 75-81 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2226
