International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (109) Last page : (114)
Article doi: http://dx.doi.org/10.18782/2320-7051.2185
Enhanced Production of Cellulolytic Enzymes from Immobilized Cells of Aspergillus niger
M. Siddiqui1*, N. Siddiqui2 and R. Varma1
1Department of Botany, SNGGPG College, Shivaji Nagar, Bhopal, M.P, india
2Department of Botany, Govt. Geetanjali Girls College, Bhopal, M.P, India
*Corresponding Author E-mail: mehar.sid@gmail.com
Received: 03.01.2016 | Revised: 15.01.2016 | Accepted: 19.01.2016
Abstract
CMCase production by immobilized Aspergillus niger in submerged fermentation was studied.The isolated culture of Aspergillus niger was immobilized in calcium alginate beads. Studies were carried out on different parameters like alginate concentration, incubation time, bead inoculum, CaCl2 concentration and curing time, which affect the productivity and stability of the immobilized system. The maximum CMCase activity of 0.37 IU/ml was achieved with 4% alginate concentration, 48h of incubation time, 40 beads/flask of inoculum, 0.2M CaCl2 concentration and 60 min of curing time.
Key words: CMCases, Calcium alginate, Aspergillus niger
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Siddiqui, M., Siddiqui, N. and Varma, R., Enhanced Production of Cellulolytic Enzymes from Immobilized Cells of Aspergillus niger, Int. J. Pure App. Biosci. 4(1): 109-114 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2185
