International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 1
First page : (240) Last page : (247)
Article doi: http://dx.doi.org/10.18782/2320-7051.2219
Antimicrobial profiling and synergistic interaction between leaves extracts of Cryptolepis buchanani (Roem. and Schult.)
A. D. Bholay1*, Abhilasha Rajguru1, Priyanka Khandale1 and Apurv Gaur2
1PG Dept. of Microbiology, K.T.H.M. College, Savitribai Phule Pune University, Nashik, Maharashtra, India
2Dept. of Microbiology, K.S.K.W. A.S.C. College, Savitribai Phule Pune University, Nashik, Maharashtra, India
*Corresponding Author E-mail: adbholay@gmail.com
Received: 14.02.2016 | Revised: 21.02.2016 | Accepted: 23.02.2016
Abstract
The following study explores the antimicrobial activity, synergistic effect, potentiation effect and phytochemical analysis of different extracts of C.buchanani leaves. C.buchanani leaves were dried and extracted by soxhlet extraction using four different solvents viz Ethyl acetate, Methanol, Acetone and Ethanol. The extracts of C.buchanani leaves were screened against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Proteus vulgaris, Xanthomonas citri, Citrobacter divergence, Vibrio cholerae, Candida albicans & Aspergillus niger. The antimicrobial activity indicated that ethyl acetate extract gave maximum inhibition followed by methanol, acetone and ethanol. Proteus vulgaris was found to be most susceptible while Aspergillus niger was resistant to all the organic extracts for antimicrobial and synergistic activity. The combinations of ethyl acetate extracts has shown highest synergistic effect against P.vulgaris. Antagonistic effect was seen for fungal pathogens. Potentiation effect of Gentamicin was seen in ethyl acetate extract against P.vulgaris. The phytochemical constituents varied significantly depending upon the type of solvent used. Saponins and alkaloids were present in all extracts. Thus, C.buchanani is highly rich in the diversity of phytochemical constituents.
Key words: C. buchanani, Antimicrobial activity, Synergistic effect, Potentiation effect, Phytochemicals.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Bholay, A.D., Rajguru, A., Khandale, P. and Gaur, A., Antimicrobial profiling and synergistic interaction between leaves extracts of Cryptolepis buchanani (Roem. and Schult.), Int. J. Pure App. Biosci. 4(1): 240-247 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2219
