International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (201) Last page : (210)
Article doi: http://dx.doi.org/10.18782/2320-7051.2238
Improved Antibacterial and Antibiofilm Activity of Plant Mediated Gold Nanoparticles using Garcinia cambogia
B. Nithya* and A. Jayachitra
1Research Scholar, Research and Development Centre, Bharathiyar University, Tamil Nadu, India
2Assistant Professor, Department of Plant Biotechnology, School of Biotechnology
Madurai Kamaraj University, Tamil Nadu, India
*Corresponding Author E-mail: nithya.hannah@gmail.com
Received: 11.03.2016 | Revised: 23.03.2016 | Accepted: 25.03.2016
Abstract
Gold nanoparticles biosynthesis is burgeoning day by day making an impact in all biological applications. Green synthesis of nanoparticle has proven to be worthy due to slower kinetics, better manipulation, and stabilization and assuring values in medicinal field due to its environmental friendly approach and also inexpensive techniques. In the present investigation gold nanoparticles, were synthesized using the aqueous leaf extract of Garcinia combogia as the reducing agent. The disposition of gold nano particles were confirmed and characterized by UV–visible spectrum, X-ray diffraction (XRD) spectroscopy, Fourier transform Infra-red spectroscopy (FTIR), Scanning electron microscopy (SEM).Further, the antibacterial activity of the synthesized gold nano particle were screened againstBacillus Subtilis, E.coli, L. Monocytogenes, Proteus vulgaris, Vibryo parahaemolyticus., The gold nanoparticles also showed effective inhibitory activity against the Bacillus licheniformis strain Daph2 with the 20 μl of concentration. Confocal laser scanning microscopic (CLSM) images demonstrated the antibioflim potentials of AuNps concentrations ranging from 20-40µg/ ml against B. licheniformis antibioflim activity.
Key words: Garcinia cambogia, Nanoparticles, Surface Plasmon resonance, XRD, SEM, CLSM.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Nithya, B. and Jayachitra, A., Improved Antibacterial and Antibiofilm Activity of Plant Mediated Gold Nanoparticles using Garcinia cambogia, Int. J. Pure App. Biosci.4(2): 201-210 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2238
