International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 2
First page : (287) Last page : (295)
Article doi: http://dx.doi.org/10.18782/2320-7051.2236
Molecular Polymorphism in Cultivated Sesamum indicum L. and its Wild Species using PCR Based Molecular Markers
C.G. Patil1*, R.B. Pujar1 and B.R. Patil2
1Department of Botany, Karnatak Science College, Karnatak University, Dharwad-580001, India
2Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, India
*Corresponding Author E-mail: patil.cg1970@gmail.com
Received: 8.03.2016 | Revised: 15.03.2016 | Accepted: 19.03.2016
Abstract
Sesame (Sesamum indicum L.) is one of the ancient oilseed crop and its oil is highly priced because of its natural antioxidants. Genus Sesamum belongs to family Pedaliaceae, which consists of 20 odd species distributed in inaccessible parts of the world especially in Africa. The only cultivated species Sesamum indicum has large number of varieties. Genetic divergence of S. indicum L. and its wild relatives is poorly understood and even the basic chromosome number in many species is still not known. The present study deals with the use of PCR based DNA markers viz., RAPD decamer primers to assess the genetic diversity of the taxa. Out of the twenty eight primers initially screened, only sixteen primers generated polymorphism among all the species. A total of 370 amplified DNA fragments were recorded and among them 364 DNA fragments were polymorphic with an average of 22.13 bands per primer. The average genetic polymorphism recorded among the species was 98.61%. Polymorphic bands were scored and analyzed using statistical software NTSYS-pc version 2.1 and dendrogram was constructed based on UPGMA for cluster analysis of cultivated taxon and its proposed progenitor along with other wild species.
Keywords: Sesamum indicum, RAPD,Unweighted Pair Group Method with Arithmetic mean, Progenitor, Genetic diversity
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Patil, C.G., Pujar, R.B. and Patil, B.R., Molecular Polymorphism in Cultivated Sesamum indicum L. and its Wild Species using PCR Based Molecular Markers, Int. J. Pure App. Biosci. 4(2): 287-295 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2236
