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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (80) Last page : (86)
Article doi: http://dx.doi.org/10.18782/2320-7051.2280

Biocontrol Activity of Bacillus subtilis Isolated from Cow Dung Against Plant Pathogenic Fungi

R. Mangalanayaki* and T. Thamizhmarai
PG and Research Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College, Sundarakkottai, Mannargudi-614 001, Tamil Nadu, India
*Corresponding Author E-mail: sivamangalamsree@gmail.com
Received: 26.04.2016  |  Revised: 10.05.2016   |  Accepted: 13.05.2016  

Plant diseases cause considerable losses in crop production and storage. Nowadays, growers still rely heavily on chemical pesticides to prevent, or control these diseases. However, the high effectiveness and ease of utilization of these chemicals can result in environmental contamination and the presences of pesticide residues on food, in addition to social and economic problems. Consequently, there is an increasing demand from consumers and officials to reduce the use of chemical pesticides.  In this context, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative. Indeed, these bio pesticides present many advantages in term of sustainability, mode of action and toxicity compared to chemical pesticides. However, in the present study the cowdung sample was collected from in an around sundarakkottai, Mannargudi Taluk, Thiruvarur district, Tamil Nadu. The totally, there is an increasing demand from consumers and officials toreduce the use of al microbial population in the cowdung was determined by serial dilution techniques. The isolated bacterial colonies are identifying as Bacillus subtilis totally four Bacillus subtilis isolates were identify and among the four strain only. Three strains possess antagonistic activity against the fungal pathogen isolated from Rhizosphere soil sample. The isolated fungal pathogens are Aspergillus niger, Aspergillus flavus and Fusarium oxysporum. The antagonistic activity was carried out by dual culture plate method and liquid broth method.

Keywords:  Cow dung, pathogenic fungi, microbial population, Rhizophere.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mangalanayaki, R. and Thamizhmarai, T., Biocontrolactivity of Bacillus subtilis Isolated from Cow Dung Against Plant Pathogenic Fungi, Int. J. Pure App. Biosci.4(3): 80-86 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2280