International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (97) Last page : (103)
Article doi: http://dx.doi.org/10.18782/2320-7051.2303

In Vitro and In Vivo Induction, and Characterization of Toxins Isolated from Beauveria bassiana

A.G. Vikhe*, N.S.Dale, R.B. Umbarkar, G.B. Labade, A.R.Savant, and A.A. Walunj
¹Research Scholar, ²Associate Professor of Entomology, ³Associate Professor of Agronomy,
⁴Assistant Professor of Botany, ⁵Assistant Professor of Plant Biotechnology,
⁶Assistant Professor of Plant Pathology and Microbiology
Department of Agril. Entomology, College of Agriculture and Agril. Biotechnology, LONI-413736
Tal- Rahata, Dist-Ahmednagar, Maharashtra India
*Corresponding Author E-mail: akshay.vikhe99@gmail.com
Received: 5.06.2016  |  Revised: 19.06.2016   |  Accepted: 23.06.2016  

Abstract
Entomopathogenic fungi produce secondary metabolites which may bio actively help fungus in its virulence toward insect hosts. Beauveria bassiana  produces several toxic low molecular compounds in vitro as well as in vivo, the most important of them is Beauvericin. The Beauveria bassiana  fungus isolated was selected for Toxin assay. Various Toxins  was obtained from surface and submerged cultures of the fungus in PDYB and PDA, culture filtrates, and in vivo conidia harvested from insect cadavers. Results indicated that in vivo fungal conidia contained the most Beauvericin, causing a higher mortality compared with in vitro fungal products in their different concentrations. Beauvericin chromatogram revealed that Beauvericin was in its greatest quantity in comparison with the other secondary metabolites of Beauveria bassiana  isolate. The impact of Beauvericin on mean and on paralysis time was in agreement with bioassay data in treated with metabolites of the insect-derived conidia.

Keywords: Beauveria bassiana , HPLC, MALDI-TOF, Toxin Identification.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vikhe, A.G., Dale, N.S., Umbarkar, R.B., Labade, G.B., Savant, A.R. and Walunj, A.A., In Vitro and In Vivo Induction, and Characterization of Toxins Isolated from Beauveria bassiana , Int. J. Pure App. Biosci.4(3): 97-103 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2303