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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 3
First page : (21) Last page : (27)
Article doi: http://dx.doi.org/10.18782/2320-7051.2296

Antioxidant, Anticancer Cell Lines and Physiochemical Evaluation of Cobra Oil

Suchitra Khunsap1*, Taksa Vesaratchapong1, Panithi Laongbao1, Lawan Chanhome1, Supranee Buranapraditkun2, Narumol Pakmanee1 and Supatsorn Boonchang1
1Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok 10330, Thailand
2Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
*Corresponding Author E-mail: sthaithumnas@yahoo.com
Received: 26.05.2016  |  Revised: 12.06.2016   |  Accepted: 15.06.2016  

In this study, the oil was extracted from depot fat of Naja kaouthia, a cobra snake. The oil components were detected by GC-MS which showed 26.29% of saturated fatty acid, 31.42% of monounsaturated fatty acid and 23.74% of polyunsaturated fatty acid. The dominant components were Palmitic acid (17.47%), Vaccenic acid (22.98%) and Linoleic acid (16.80%). Biological functions of cobra oil were investigated for anti-oxidant and anti-cancer cells. Various concentrations of cobra oil (20%, 10%, 5%, 2.5% and 1.25%; v/v) could inhibit free radical scavenging by DPPH method 88.11%±1.62%, 54.84%±2.63%, 35.10%±6.41%, 14.61%±2.37% and 6.01%±3.41% respectively. In addition, KATO-III, HepG2 and SW620 cells undergo cytotoxic activity by cobra oil for 24, 48 and 72 h detected following MTT assay. As a great IC50 of cobra oil on KATO-III, HepG2 and SW620 were 1.63% ± 0.08% (at 72 h), 1.67% ± 0.22% (at 48 h) and 1.67% ± 0.43% (at 48 h), respectively.

Keywords: Anticancer, Antioxidant, Cobra oil, Fatty acid profile, Natural product.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Khunsap, S., Vesaratchapong, T., Laongbao, P., Chanhome, L., Buranapraditkun, S., Pakmanee, N. and Boonchang, S., Antioxidant, Anticancer Cell Lines and Physiochemical Evaluation of Cobra Oil, Int. J. Pure App. Biosci.4(3): 21-27 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2296