International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (84) Last page : (92)
Article doi: http://dx.doi.org/10.18782/2320-7051.2344
Bacterial Lignin Peroxidase in Biobleaching of Lignin-mimicking Indicator Dyes
Priyanka Uttamrao Jadhav1*, Bholay A.D.2 and Mahesh Shindikar3
1Environmental Science Research Centre, K.T.H.M. College, Nasik, S.P. Pune University, MS, India
2P.G. Dept. of Microbiology, K.T.H.M College, Nashik, S.P. Pune University, MS, India
3Department of Applied Science, College of Engineering, Pune, MS, India
*Corresponding Author E-mail: enjoypiyu@gmail.com
Received: 29.07.2016 | Revised: 8.08.2016 | Accepted: 11.08.2016
Abstract
Lignin, a complex biopolymer and textile dyes are the major pollutants of industrial waste-water and responsible for its intense undesirable dark-brown color. Due to complicated structure and non-hydrolysable bonds, lignin and some lignin-mimicking dyes are resistant to degradation. Potential applications utilizing lignin-degrading bacteria and enzymes like lignin peroxidase have become attractive because they may provide environmental friendly method for waste-water treatment of various industries. The growth on polymeric lignin or lignin monomers is not necessarily only a measure of the ligninolytic potential of bacteria.
The present work represents isolation, screening, acclimatization and identification of lignolytic and dye-decolorizing bacteria. The isolates were screened for their lignolytic and dye decolourization activity quantitatively by spectrophotometry and qualitatively by solid-phase plate assay. The approach revealed biobleaching of various dyes by bacterial lignin peroxidase. The isolate Corynebactrium jeikeium was found to decolorize indicator dyes Methylene Blue, Toluidine Blue, Congo Red and Malachite Green up to 76.32%, 64.10%, 96.51% and 81.78% respectively while Sphingomonas paucimobilis efficiently removed dyes Malachite Green, Methylene Blue and Congo Red up to 90%, 60% and 96% respectively when applied as axenic culture. The complexity of the dye structure was found to have an influence on the decolorization rate by LiP. The diverse structures of dyes might have affected the approach of ligninase (Lignin Peroxidase) cation radicals towards dye molecules. Each dye molecule contained a chromophore and its color disappeared only after the chromophore structure was destroyed by many attacks of LiP. Thus, the bacterial LiP can be adopted as an efficient tool for biobleaching of indicator dyes for small as well as large scale applications.
Key words: Biobleaching, Dye-decolourization, Lignin Peroxidase, Lignin Mimicking Dye.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Jadhav, P.U., Bholay A.D. and Shindikar, M., Bacterial Lignin Peroxidase in Biobleaching of Lignin-mimicking Indicator Dyes, Int. J. Pure App. Biosci.4(4): 84-92 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2344
