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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (102) Last page : (113)
Article doi: http://dx.doi.org/10.18782/2320-7051.2324

Microbiological, Pharmacological and Phytochemical Efficacy of Catharanthus roseus (L.) G. Don

Apurv Gaur1*, Malarvili Ganeshan2, Rameez Shah3 and A.D. Bholay4
1Department of Microbiology, K.S.K.W. A.S.C. College, Savitribai Phule Pune University, Nashik, M.H., India
2NADIR Godrej Centre for animal research and development, Peth Road, Dindori, Nashik, M.H., India
3Department of Health Care Administration, Maharashtra University of Health Sciences, Nashik, M.H., India
4PG Department of Microbiology, K.T.H.M. College, Savitribai Phule Pune University, Nashik, M.H., India
*Corresponding Author E-mail: gaur.apurv@gmail.com
Received: 30.06.2016  |  Revised: 8.07.2016   |  Accepted: 10.07.2016  

Abstract
Madagascar periwinkle (Catharanthus roseus) is a medicinal plant of ethnopharmacological importance. The antimicrobial activity of all the extracts of C. roseus against S. typhi, X. citri, S. dysentriae, S. paratyphi B, A. niger and V. cholerae along with potentiation effect on Gentamicin is reported for the first time in literature to the best of our knowledge. ¬†Organic solvents with different polarity and dipole moment were used to extract the bioactive compound from dried leaves of C. roseus using soxhlet extraction. Extracts were evaluated for their antimicrobial susceptibility, synergistic and potentiation effect against the tested pathogens and their phytochemical contents were also analysed. Data interpretation indicates that the pattern of inhibition depends upon the plant part used, the extraction procedure, temperature, the assaying method and the organisms tested. It has been reported for the first time that polarity and dipole moment of a solvent plays an important part in the extraction of bioactive compound which in turn determines various activities being examined. X. citri was found to be most susceptible, while A. niger was resistant to all the organic extracts for the antimicrobial and synergistic activity. The combination of extracts tested for synergistic activity against the tested pathogens was found to be additive for bacterial pathogens and antagonistic in case of fungal pathogens. Blend of extracts with ethyl acetate was found to give better synergistic activity. Phytochemical analysis revealed alkaloids in all the extracts. Saponins, tannins and phytosterols were also present in some extracts. Activity of acetone extract was found to be maximum than ethyl acetate, followed by methanol, ethyl alcohol and chloroform.

Keywords: C. roseus, Antimicrobial activity, Potentiation effect, Synergistic effect, Phytochemicals.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782


Cite this article: Gaur, A., Ganeshan, M., Shah, R., Bholay, A.D., Microbiological, Pharmacological and Phytochemical Efficacy of Catharanthus roseus (L.) G. Don, Int. J. Pure App. Biosci.4(4): 102-113 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2324