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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (114) Last page : (122)
Article doi: http://dx.doi.org/10.18782/2320-7051.2342

Effect of Organophosphorus Insecticide, Malathion on the Division of Meristems of Allium cepa L.

Pari Gogoi1, Saurav Das2*, Sayak Das2 and Mohammad Zeeshan Alam Khan2
1Department of Life-Sciences, Dibrugarh University, Dibrugarh – 786004, Assam, India
2Department of Microbiology, Sikkim University, Gangtok – 737102, Sikkim, India
*Corresponding Author E-mail: saurav12das@gmaiL.com
Received: 25.07.2016  |  Revised: 3.08.2016   |  Accepted: 5.08.2016  

The objective of the present work is to study the effect of an organophosphorus insecticide (Malathion) on the division of meristems of Allium cepa. l. Fresh healthy onion bulbs were treated with different concentration of Malathion from 0.1% - 0.9%. Treated bulbs were sliced into transverse section and mitotis slides were prepared by Acetocarmine hydrolysis technique to observe the changes in mitotic phases under a microscope. A series of mitotoxicity was observed under the influence of Malathion. The abnormal mitotic phases can be clearly observed in the disturbed metaphase and anaphase and shrinkage of anaphase chromosomes. In, 0.1%, 0.2% and 0.5% concentration the chromosomes got separated and loosened, clearly avoiding the pair formation and in some instances, spindle formation was inhibited. The maximum mitotic division was observed in Treatment 1 (0.1%) and Treatment (0.2%). The initial mitotic depression was observed in Treatment 4 (0.4% concentration) which gradually increased with increasing concentration. Highest mitotic depression was observed in Treatment 9 (0.9%) with a Mitotic Index (MI) of 11%. Malathion can be mitotoxic for plant causing aberration in different mitotic phases and which can arrest cellular growth and leads to senescence.

Key words: Malathion, Allium cepa L., Mitotoxic Organophosphorus, Mitosis, Metaphase

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Gogoi, P., Das, S., Das, S.  and Khan, M.Z.A., Effect of Organophosphorus Insecticide, Malathion on the Division of Meristems of Allium cepa L., Int. J. Pure App. Biosci.4(4): 114-122 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2342