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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (205) Last page : (211)
Article doi: http://dx.doi.org/10.18782/2320-7051.2357

Antibacterial Activity of Evolvulus nummularius Against Standard ATCC Gram Positive and Gram Negative Strains: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation

Susmita Saha1, Bijit Deb1, Jhinuk Basu Mullick1, Parichita Ray Choudhury1, Partha Saha1, Biplab Ghosh2 and Samir Kumar Sil1*
1Molecular Genetics and Cell Physiology Lab., Department of Human Physiology, Tripura University, Suryamaninagar, West Tripura-799022, India
2Department of Chemistry, Netaji Subhash mahavidhyalaya, Udaypur, Gomati Tripura-799022, India
*Corresponding Author E-mail: hitaishi1990@gmail.com
Received: 15.08.2016  |  Revised: 22.08.2016   |  Accepted: 24.08.2016  

Evolvulus nummularius is a widely used ethnomedicinal plant of North-East India. The whole plant of E. nummularius is used as a medicine for hysteria, to cure burns, cuts, wounds and scropion stings. This plant has antihelminthic activity, wound healing activity, poor sedative and anticonvulsant properties. In this study, the antibacterial activity of crude methanol extract of E. nummularius was studied against three standard gram positive and two gram negative strains of bacteria (ATCC strains). This plant extract showed both bacteriostatic and bactericidal activity against all bacterial strains. The extract was most active against gram positive bacterial strain S. pyogenes and B. subtilis.  In growth analysis study, it was obtained that, after treatment with IC50 dose for each bacterial strains, the lag phase become extended compared to untreated bacterial cells. To understand the mechanism of action of Evolvulus nummularius the reactive oxygen species (ROS) was estimated and result showed that, reactive oxygen species were increased (40-55%) in presence of IC50 dose of Evolvulus nummularius.

Key words: Evolvulus nummularius, Gram-positive bacteria, Gram negative bacteria, Growth curve, ROS.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Saha, S., Deb, B., Mullick, J.B., Choudhury, P.R., Saha, P., Ghosh, B., and Sil, S.K., Antibacterial Activity of Evolvulus nummularius against Standard ATCC Gram Positive and Gram Negative Strains: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation, Int. J. Pure App. Biosci.4(4): 205-211 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2357