International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (212) Last page : (220)
Article doi: http://dx.doi.org/10.18782/2320-7051.2321

Optimization of Lactase Production under Submerged Fermentation by Lactobacillus sp. KLSA 22

Sarfaraz Ahmed, Lingappa Kattimani*, Mahesh Divatar, Sandhya Gajare, Shivalee A, Asma Farheen and Masarath Irfana
Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi- Karnataka, India
*Corresponding Author E-mail: lingappak2@gmail.com, lingappak1@rediffmail.com
Received: 28.06.2016  |  Revised: 10.07.2016   |  Accepted: 12.07.2016  

Abstract
Lactase yield was determined by inoculating Lactobacillus sp. KLSA 22 under optimized conditions of fermentation. Investigation results showed that maximum lactase activity (97.99 ± 02.38 IU) with 0.5% substrate (lactose in cheese whey) concentration at static condition.  In addition, the studies of highest enzyme activity were observed at pH 6.5 and temperature 35°C at 48 h of incubation period. Under this optimal condition nitrogen source and metal ions used, yeast extract and MgSO4 was found to favor the enzyme production respectively. The organism could be used for the commercial production of lactase for various applications in industrial scale. 

Key words: β-galactosidase, lactose, whey, enzyme production, Latobacillus sp. and ONPG.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sarfaraz, A., Lingappa K., Divatar, M., Gajare, S., Shivalee A., Farheen, A. and Irfana, M., Optimization of Lactase Production under Submerged Fermentation by Lactobacillus sp. KLSA 22,       Int. J. Pure App. Biosci.4(4): 212-220 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2321