International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (264) Last page : (274)
Article doi: http://dx.doi.org/10.18782/2320-7051.2329

Bioconversion of Waste Paper into Bio-Ethanol by Co-Culture of Fungi Isolated from Lignocellulosic Waste

Payal Varotkar, P. M. Tumane and Durgesh D. Wasnik*
Post Graduate Department of Microbiology, L.I.T. Premises, R.T.M. Nagpur University, Nagpur (M.S.) India
*Corresponding Author E-mail: dwasnik75@gmail.com
Received: 2.07.2016  |  Revised: 11.07.2016   |  Accepted: 13.07.2016  

Abstract
In present study, examined the relative potentials of waste paper as microbial substrate for sacchrification using wild strains of Aspergillus sp., Penicillium sp. and Trichoderma sp. isolated from different degraded cellulose waste material. Office paper showed the strongest susceptible for hydrolysis by Aspergillus sp. (3.76mg/ml) followed Trichoderma sp. (3.26mg/ml) and Penicillium sp. (2.6mg/ml), On the other hand, news paper and filter paper showed least reduction of sugar by tested fungi. Saccharification of fungal cultures were mixed and incubated with all cellulosic materials. An equal mixture 2 (2:2:2) resulted in the highest increase in saccharification with office paper, filter paper as compared to mixture 1. The highest cellulase produced from office paper by Penicillium sp. followed by news paper and filter paper and highest cellulase also produced from office paper by mixed culture 2 followed by filter paper and news paper. The highest rate of protein were produced from news paper by Penicillium sp. and then Aspergillus sp., Trichoderma sp. and the highest rate of protein were also produced from news paper by mixed culture 2 than mixed culture 1 followed by office paper and filter paper. Office paper selected for the production of bioethanol for highest sacchrification by
A. niger. The maximum amount of Ethanol estimated using Potassium dichromate method was 1.3ug/ml which was produced from 5gm office paper saccharified by Aspergillus sp.

Key words: Waste paper, Saccharification, co-culture, Fungus, lignocellulosic waste

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Varotkar, P., Tumane, P.M. and Wasnik, D.D., Bioconversion of Waste Paper into Bio-Ethanol by Co-Culture of Fungi Isolated from Lignocellulosic Waste, Int. J. Pure App. Biosci.4(4): 206-215 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2329