International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (309) Last page : (315)
Article doi: http://dx.doi.org/10.18782/2320-7051.2354

Detection of Seed Borne Myco-Flora Associated with Chickpea

S.N. Warude, S.S. Mane* and G.K. Giri
Department of Plant Pathology, PGI, Dr.PDKV, Akola (M.S.) 444101, India
*Corresponding Author E-mail: drssmane68@gmail.com
Received: 13.08.2016  |  Revised: 20.08.2016   |  Accepted: 22.08.2016  

Abstract
The seed borne myco-flora of 10 varieties of chickpea, Vijay, Jaki-9218, ICCC-37, Digvijay, PKV Kabuli-2, Vihar, Virat, Saki-9516, AKGS-1 and Gujarat-1 collected from different location of Maharashtra were examined by blotter method ,agar plate method, deep freezing and 2-4 D method as recommended by ISTA. A total of six genera of fungi viz. Alternaria alternata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium spp. Rhizopus spp., Curvullaria lunata and Rhizoctonia bataticola were reported. Among them Aspergillus niger, Aspergillus flavus, Fusarium oxysporum and Rhizopus spp. were predominant to all seed samples. Amongst the methods used for detection of seed borne fungi, the blotter paper method is more effective followed by agar plate method, deep freeze method and 2, 4-D method.

Key words: Chickpea, seed borne mycoflora, blotter paper method, agar plate method, deep freeze method, 2, 4-D method.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Warude, S.N., Mane, S.S. and Giri, G.K., Detection of Seed Borne Myco-Flora Associated with Chickpea, Int. J. Pure App. Biosci.4(4): 309-315 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2354