International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 4
First page : (50) Last page : (68)
Article doi: http://dx.doi.org/10.18782/2320-7051.2322
Integrated Assessment of Phytostimulation and Biocontrol Potential of Endophytic Trichoderma spp Against Common Bean (Phaseolus vulgaris L.) Root Rot Fungi Complex in Centre Region, Cameroon
Eke Pierre1, Nana Wakam Louise1,2*, Toghueo Kouipou Rufin Marie1, Tsouh Fokou Patrick Valère1, Jesus Marie Arc-en-ce1 and Boyom Fabrice Fekam1
1Antimicrobial & Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I,
P.O. Box: 812, Yaoundé, Cameroon
2Laboratory of Soil Microbiology, Biotechnology Centre, Nkolbisson, University of I, Cameroon
*Corresponding Author E-mail: wnanalouise@yahoo.fr
Received: 29.06.2016 | Revised: 14.07.2016 | Accepted: 17.07.2016
Abstract
This study aimed to assess the growth promotion and the in-vitro and greenhouse efficacy of Trichoderma endophytes from bean root against Fusarium oxysporum f.sp. phaseoli, Fusarium solani f.sp. phaseoli and Macrophomina phaseolina, causing root rot in Cameroonians bean (phaseolus vulgaris l.) fields. From twelve Trichoderma endophytes, Trichoderma polysporum T1, Trichoderma atroviridae T2, Trichoderma sp. T3 and Trichoderma harzianum T8 were selected upon in vitro antifungal screening (up to 88.2% growth inhibition). The selected bioagents could synthetise and release proteolytic enzymes and phosphate solubilization factors. T. harzianum T8 and T. polysporum T1 released the most active volatile and non-volatile antifungal metabolites, which together with conidia had either inhibitory or stimulating effects on bean seeds germination. In greenhouse, T. hazianum T8 (94.16%) andT. sp T3 (74.16%) enhanced seed germination velocity and percentage as well as shoot elongation (71.8 cm) more than otherisolates both in absence and the presence of F. solani. A significant reduction in disease indexes was registred with the overall biocontrol agents, the maximum being recorded with Trichoderma sp. T3 (up to 50%). This further highlight the understanding on either the pathogenic nature of Trichoderma isolates or the improvement of bean resistance through direct and/or indirect action on causal pathogens.
Key words: Phaseolus vulgaris L. F.oxysporum, F. solani, M. phaseolina, Trichoderma spp, biocontrol.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Pierre, E., Louise, N.W., Marie, T.K.R., Valère, T.F.P., Arc-en-ce, J.M. and Fekam, B.F., Integrated Assessment of Phytostimulation and Biocontrol Potential of Endophytic Trichoderma spp Against Common Bean (Phaseolus vulgaris L.) Root Rot Fungi Complex in Centre Region, Cameroon, Int. J. Pure App. Biosci.4(4): 50-68 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2322
