International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 5
First page : (153) Last page : (159)
Article doi: http://dx.doi.org/10.18782/2320-7051.2372
Isolation, Characterization, Identification and Screening of Amylase Producing Bacteria from Soil under System of Rice Intensification (SRI) Technique
Shruti* and P. Banik
Department of Agriculture and Ecological Research unit, Department of Human Genetic
Indian Statistical Institute, Kolkata, W.B., India
*Corresponding Author E-mail: shruti.mona007@gmail.com
Received: 8.09.2016 | Revised: 20.09.2016 | Accepted: 21.09.2016
Abstract
Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. In the present investigation, bacteria were isolated from rhizosphere of Rice Agricultural Experimental Farm, Giridh, Jharkhand, screened for the production of amylase and their optimum growth conditions were determined. A total of 36 bacterial colonies were isolated from collected soil samples. Thirty Four isolates are from geneus Bacillus and two are paenibacillus, out of these five bacterial isolates, displayed zones of clearance in starch hydrolysis test. The isolate displaying maximum amylase activity on quantization was selected. Characteristic feature of the strain indicates that it belongs to the genus Bacillus and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The optimum pH for enzyme activity was found to be at pH 7.0 and the optimum temperature for the activity was found to be at 40oC.
Key words: Amylase, Starch Hydrolysis, characterization, amylase activity
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Shruti and Banik, P., Isolation, Characterization, Identification and Screening of Amylase Producing Bacteria from Soil under System of Rice Intensification (SRI) Technique, Int. J. Pure App. Biosci.4(5): 153-159 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2372
