International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 5
First page : (168) Last page : (174)
Article doi: http://dx.doi.org/10.18782/2320-7051.2383

Evaluation of Genotoxicity in Tannery Industrial Workers Exposed to Chromium using Comet Assay

K. Rudrama Dev*i
Human Genetics and Molecular  Lab,  Department of Zoology,University College of Science, Osmania University, Hyderabad – 07., A.P., India
*Corresponding Author E-mail: rudramadevi_k@yahoo.com
Received: 1.10.2016  |  Revised: 18.10.2016   |  Accepted: 21.10.2016  

ABSTRACT

A study was conducted on a group of employees from tannery industries in Telangana state were tested for comet tail lengths that are well established as indicators of early biological effects. To investigate whether occupational exposure to chromium is genotoxic, a total of 92 workers and 80 control groups of individuals in the age group of 16 to 65 years and 6-30 yrs of chromium exposure were recruited; a questionnaire based survey was conducted. In the present study, the assessment of Primary DNA damage hosted by peripheral blood leukocytes of workers employed in tannery based industry was performed using the alkaline comet assay, the tail length and long-tailed nuclei thereby being the primary outcome of the measure. A significant increase in the incidence of DNA damage was observed in the experimental subjects when compared to their respective controls. The results obtained in this investigation indicate that tannery workers seem to be facing the occupational hazard of genotoxicity due to inhalation and handling of chromium.

Key words: DNA, Genotoxicity, Assay, Chromium

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Rudrama, K.D. Evaluation of Genotoxicity in Tannery Industrial Workers Exposed to Chromium using Comet Assay, Int. J. Pure App. Biosci. 4(5): 168-174 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2383