International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 5
First page : (236) Last page : (243)
Article doi: http://dx.doi.org/10.18782/2320-7051.8228
Callus induction in wide cross of Brassica
Chandan Kishore1*, Awadhesh Kumar Pal2, Kundan Kishor Rajak3, Taraknath Goswami4,
Anil Kumar1 and Shashikant2
1Department of Plant Breeding and Genetics, 2Department of Plant Physiology and Biochemistry,
4Department of Entomology, 4Department of Plant Pathology
Bihar Agricultural University, Sabour, Bhagalpur-813210
3Department of Agricultural Biotecnology and Molecular Biology,
Rajendra Agricultural University, Pusa, Samastipur- 848125, India
*Corresponding Author E-mail: chandanpbg@gmil.com
Received: 10.09.2016 | Revised: 17.10.2016 | Accepted: 24.10.2016
ABSTRACT
Utilisation of naturally occurring variability of cultivated species in Brassica is limited due to its narrow genetic base. The pre-breeding approaches for introgression of crucial genes from wild to cultivated species faces barriers in generation of wide hybrids. In such a situation, embryo rescue has proved to be one of the best tools for generation of distant hybrids. The present study was carried out with the objective to standardise the media composition for the rescue of hybrid embryo obtained through the inter-specific crosses between Brassica fruticulosa (FF, 2n =16) and Brassica rapa (AA, 2n = 20). Pistils of crosses obtained after 3 to 5 days of pollination were used as explants. Out of three media compositions MS media supplemented with casein hydrolysate and benzo-aminopurine reflected the best result for ovary and ovule culture to revive the embryo. The well established pistils showed more than eighty per cent swelling of cells. The axially organized growth of cells was observed in the swelled explants and later it was converted into a shapeless bulk of living matter. After 21 days of inoculation, growth of cell mass appeared in the form friable and greenish cream callus. About sixty percent callogenesis was observed. The optimised media compositions may be useful in generation of wide cross of Brassica and related crops.
Key words: Brassica fruticulosa and Brassica rapa, Callogenesis, Embryo rescue, Mustard
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kishore, C., Pal, A.K., Rajak, K.K., Goswami, T., Kumar, A. and Shashikant, Callus induction in wide cross of Brassica, Int. J. Pure App. Biosci. 4(5): 236-243 (2016). doi: http://dx.doi.org/10.18782/2320-7051.8228
