International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 5
First page : (244) Last page : (248)
Article doi: http://dx.doi.org/10.18782/2320-7051.8229
Clonal Fidelity of In Vitro Propagated Orchid Cultivars Dendrobium ‘Sonia’ and Dendrobium nobile var. 'Emma White’
Kamni Kumari1, Kundan Kishor Rajak1, Chandan Kishore2* and Mithilesh Kumar1
1Department of Agricultural Biotecnology and Molecular Biology,
Rajendra Agricultural University, Pusa, Samastipur- 848125, India
2Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour, Bhagalpur-813210, India
*Corresponding Author E-mail: chandanpbg@gmil.com
Received: 16.09.2016 | Revised: 21.10.2016 | Accepted: 27.10.2016
ABSTRACT
Micropropagated plantlets of two orchid cultivars namely Dendrobium ‘Sonia’ and Dendrobium nobile var. 'Emma White’ were analyzed for genetic fidelity by RAPD markers. The in vitro raised plantlets were maintained repeatedly by in- vitro subcultures. Out of 20 primers utilized during present investigation, amplification was achieved with all primers. But reproducible amplification protocol was standardized only for 8 primers namely, OPL-02, OPL-03, OPL-8, OPL- 11, OPN-01, OPN-06, OPN-09, OPN-10. The amplified products were monomorphic across all the selected micropropagated plants and were similar to the mother plant. So, the RAPD analysis of micro propagated plant along with their original cultivar with eight decamer primers did not showed any variation. Hence, it confirmed the genetic uniformity of micropropagated plantlets in the present study.
Key words: Orchid, Tissue Culture, MS media, RAPD
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Kumari, K., Rajak, K.K., Kishore, C. and Kumar, M., Clonal Fidelity of In Vitro Propagated Orchid Cultivars Dendrobium ‘Sonia’ and Dendrobium nobile var. 'Emma White’, Int. J. Pure App. Biosci. 4(5): 244-248 (2016). doi: http://dx.doi.org/10.18782/2320-7051. 8229
