International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (186) Last page : (192)
Article doi: http://dx.doi.org/10.18782/2320-7051.2409

In Vitro Effect of Various Plant Growth Promoters on Clerodendrum serratum

Rahul Vijay*, Jyoti Shukla and Rajesh Saxena
Madhya Pradesh Council of Science and Technology, Bhopal (M.P) India
*Corresponding Author E-mail: drrahulvijay001@gmail.com
Received: 24.11.2016  |  Revised: 6.12.2016   |  Accepted: 10.12.2016  

Abstract
Background and Aim: In-vitro effect of various growth regulators on propagation of Clerodendrum serratum, a medicinally herbs of India was observed in this study. C. serratum is an important medicinal plant and used in the various ancient traditional system of medicine.
Materials and Method:Healthy nodal segments of the herb was used as explants with basic MS medium for shoot initiation and multiplication containing various combinations of different growth regulators. MS full and 1/2MS were used for rooting of plantlets with 25 to 200 mg/l Activated Charcoal.
Result: Maximum mean number of proliferated plantlets 1.4 ± 0.84 with mean length 3.5 ± 2.01 were found in MS medium treated with lowest concentration of BA, while maximum mean number of multiplied plantlets 22.8 ± 1.75 with mean length 5.3 ± 1.15 were found in MS medium treated with 6.66 µM. Maximum mean number of roods 11.6 ± 1.17 with Mean length 9.01 ± 0.57 was observed on 1/2MS medium with 100 mg/l activated charcoal. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully established in soil.

Key words: Clerodendrum serratum, Micropropagation, Medicinal Plants, Plant Tissue Culture.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Vijay, R., Shukla, J. and Saxena, R., In Vitro Effect of Various Plant Growth Promoters onClerodendrum serratum, Int. J. Pure App. Biosci.4(6): 186-192 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2409