International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (16) Last page : (26)
Article doi: http://dx.doi.org/10.18782/2320-7051.2387
High Frequency of Multiple Autoantibody Positive LADA in Patients with Type 2 Diabetes Mellitus in Iran Population
Mohammad Khoshroo1*, Mehdi Shekarabi1,2, Mohammad Ebrahim Khamseh4, Naser Kalhor5, Leila Novin6, Zhra Shiri7, Mohammad Khazeni8 and Mehdi Yusefi6
1Department of Medicine, Faculty of Medicine, Qom Branch, Islamic Azad University, Qom, Iran
2Department of Immunology, Faculty of medicine, Iran University of Medical Sciences, Tehran, Iran
3Immunology research center, Iran University of Medical Sciences, Tehran, Iran
4Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Firouzgar alley, Valadi St., Behafarin St., Karimkhan Ave., Vali-asr Sq., Tehran, Iran
5Department of biology ACCER, Qom Branch, Iran
6Department of Medicine, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
7Department of Biology, Payam-e-Noor University, Tehran, Iran
8Department of microbiology and Immunology, school of Medicine, Qom University of Medical Sciences, Qom, Iran
*Corresponding Author E-mail: mohammad.khoshroo@yahoo.com
Received: 8.10.2016 | Revised: 20.10.2016 | Accepted: 23.10.2016
Abstract
Background: Latent autoimmune diabetes of adults (LADA) is a slowly progressive form of autoimmune diabetes initially managed with diet and oral hypoglycemic agents before becoming insulin requiring. Autoantibodies are used for differential diagnosis between LADA and Type 2 Diabetes Mellitus. We assess the frequency of LADA using islet autontibodies in Qom, Iran population with T2DM and characterize clinical and biochemical characteristics of them.
Methods: In this cross-sectional study, 86 clinically diagnosed as T2DM patients aged between 27-73 years were tested for presence of autoantibodies by ELISA technique. The GADA, ICA and IA2A assessment are used for diagnosis of LADA patients.
Results: Of 86 phenotypically T2DM, 35(40.70 %) were antibody positive and 51(59.30%) persons were negative for any antibody. A significance difference was found between LADA and antibody negative T2DM patients groups in age, duration of disease, family history of diabetes, autoimmune thyroid disease and C- peptide levels. Altogether 21(60.0%) of patients had two or more antibodies, 6(17.14%) were only GADA positive, 7(20%) patient were single ICA positive and 1(2.86%) patient was single IA2A positive. None of the sera were positive for ICA and IA2A in combination.
Conclusions: Among our T2DM subjects, islet autoantibody positive patients were not uncommon. Also, multiple autoantibodies were more frequent than single autoantibodies in LADA patients. Seroconversion after disease progress and our methodology can explain our observation.
Key words: LADA, Autoantibody, T2DM.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Khoshroo, M., Shekarabi, M., Khamseh, M.E., Kalhor, N., Novin, L., Shiri, Z., Khazeni, M. and Yusefi, M., High Frequency of Multiple Autoantibody Positive LADA in Patients with Type 2 Diabetes Mellitus in Iran Population, Int. J. Pure App. Biosci.4(6): 16-26 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2387
