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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (27) Last page : (46)
Article doi: http://dx.doi.org/10.18782/2320-7051.2413

Simultaneous Detection of Tricyclic Antidepressants using Higher Performance Liquid Chromatography

Jamiu Mustapha Sulayman1* and Bashir Abdulkadir2
1Department of Anatomy, Faculty of Health Sciences University of Ilorin, Nigeria
2Department of Microbiology, Umaru Musa Yar’adua University Katsina, Nigeria
*Corresponding Author E-mail: jamiu.sulayman@yahoo.com
Received: 29.11.2016  |  Revised: 14.12.2016   |  Accepted: 18.12.2016  

Despite the introduction of newer antidepressants, tricyclic antidepressants are still widely used for the treatment of major depression, chronic pain and anxiety.  And is the most leading cause of drug-induced death in many patients. Monitoring their blood/plasma concentration is therefore a priority in forensic toxicology. In this study, a quick and rapid method for simultaneous detection and quantification of three TCAs (desipramine, nortriptyline and amitriptyline) in whole blood was developed and validated on HPLC-UV under a gradient elution, using a C18 column and a mobile phase mixture of ACN (35% v/v) and a pH 3.5, 50mM phosphate buffer (65% v/v). The evaluated validation parameters were satisfactorily accepted in accordance with ICH and FDA guidelines. The determination coefficients were more than 0.99 within the tested concentration range (0.1–1mgL-1), LOD and LOQ were between 0.0027-0.0051 and 0.27-0.051 respectively, Accuracy expressed as % Er was found to be between 0.1-7% and precision expressed as %CoV was found to be between 2-10%. All compounds were satisfactorily determined in one single injection within 20 min. sample pre-treatment was optimised on three sets of cartridges (C2,C18 and Strata-X) on a solid phase extractions, with C18  giving the best recovery for all analytes ranging from 60-105%. The developed method proved to be suitable for routine work and it was used to successfully analyse forensic blood samples.

Key words: Antidepressants, HPLC, tricyclic, toxicology and simultaneous.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Sulayman, J.M. and Abdulkadir, B., Simultaneous Detection of Tricyclic Antidepressants using Higher Performance Liquid Chromatography, Int. J. Pure App. Biosci.4(6): 27-46 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2413