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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2016, Volume : 4, Issue : 6
First page : (47) Last page : (58)
Article doi: http://dx.doi.org/10.18782/2320-7051.2414

Insilico Strategy of Epitope Design in Clostridium botulinum

Khalida Naveed*, Naila Jawaid and Syeda Tatheer Fatima
Baqai Institute of Information Technology, Baqai Medical University, Karachi, Pakistan
*Corresponding Author E-mail: khalidanaveed@baqai.edu.pk
Received: 30.11.2016  |  Revised: 15.12.2016   |  Accepted: 17.12.2016  

Clostridium botulinum is the name of a collection of bacteria that frequently habitat in soil. These are rod-shaped organisms that rose under anaerobic condition. They have the ability to produce spores for their survival under unfavorable conditions. The C. botulinum genome comprises of 3,886,996 BP, 28.24, GC%, 3650 coding sequence, 9rRNA and 80tRNA. Identifying a legend in a drug discovery project assumes that the drug target is known and has been characterized. The majority of available drugs have protein molecules as their targets for botulinum toxins are available for botulinum toxins. In our current project, potential epitope were identified in C. botulinum genome (An epitope is the part of the antigen that binds to a specific antigen receptor on the surface of a B cell) the predicted epitope by using available bioinformatics tools i.e. Vaxijen, TMHMM, Bcpred, MHCpred, Propred I, Propred, T-epitope designer, phyre and Pepitope. The two screening steps were adopted in this work. Previously identified 22 essential membrane proteins sequence were taken and in the first step screening the Vaxijen, TMHMM and Bcpred were used for selecting C. botulinum proteins as antigenic exo-membrane B-epitope.  The two epitope, one from each protein have been designed in such a way that each epitope is highly likely to bind determined the number of (HLA molecules comprising of both the MHC-I and II) and interacts with most frequent HLA alleles (A*0201, A*0204, B*2705, DRB1*0101, and DRB1*0401) in human population. Therefore, our selected epitope are highly potential to induce both the B-cell and T-cell mediated immune responses. Of course, these selected epitope require further experimental validation.

Key words:  insilico strategy,epitope prediction, Clostridium botulinum.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Naveed, K., Jawaid, N. and Fatima, S.T., Insilco Strategy of Epitope Design in Clostridium botulinum, Int. J. Pure App. Biosci.4(6): 47-58 (2016). doi: http://dx.doi.org/10.18782/2320-7051.2414