International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 1
First page : (135) Last page : (141)
Article doi: http://dx.doi.org/10.18782/2320-7051.2449
Efficient Callus Induction and Regeneration in Brassica juncea for Environment Friendly Agriculture
Javeed Ahmad Lone1*, Surinder Kumar Gupta2, Shabir Hussain Wani3, Mamta Sharma2 Rayees Ahmad lone1 and Asif Bashir Shikari4
1,3Division of Genetics and Plant Breeding, FoA, Wadura Sopore 193201, SKUAST-Kashmir,
2Sher-e- Kashmir University of Agricultural Science and Technology Chatha-190008 Jammu, Division of Genetics and plant Breeding
4Sher-e- Kashmir University of Agricultural Science and Technology Shalimar-190025, Division of Plant Biotechnology SKUAST- Kashmir
*Corresponding Author E-mail: javeedlone09@gmail.com
Received: 12.01.2017 | Revised: 20.01.2017 | Accepted: 23.01.2017
ABSTRACT
In the present study an efficient regeneration protocol has been established for mustard (Brassica juncea var RSPR 01, RSPR 03) using hypocotyls of in vitro grown seedlings for callus induction and regeneration. Different concentrations of phytohormones, auxin (NAA: Naphthalene acetic acid, 2,4-Dichlorophenoxyacetic acid) and cytokinins (BAP: Benzylaminopurine) were used for callus induction and plant regeneration. Formation of callus from hypocotyls explants were observed in the MS media supplemented with 3% sucrose and 2,4-Dichlorophenoxyacetic acid at different concentrations of (0.5, 1.0, 1.5, 2.0 and 2.5 mg/l) high response of about 57.14 to 95.24% was observed at 2.0 to 2.5 and no response was found at 0.5 and 1.0 mg/l of 2,4 –D. The regeneration frequency was observed with different concentrations of (MS+ 2,4-D (0.5mg/l)+BAP (1, 2, 3, 4 and 5 mg/l) the concentration of 2,4D were kept constant with the change in concentration of BAP from 1.0 to 5.0mg/l. Regeneration frequency (RF) of 100 per cent with variety RSPR 03 was observed in the media supplemented with 0.5mg/l 2,4-D +5.0 mg/l BAP while as the cultivar RSPR 01 showed regeneration frequency of 100 per cent when 0.5mg/l 2,4-D +4.0 mg/l BAP. This protocol can be further explored for transformation of mustard for incorporation of specific genetic traits for its improvement.
Key words: Brassica juncea, Callus induction, Regeneration efficiency, Protocol.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Lone, J.A., Gupta, S.K., Wani, S.H., Sharma, M. lone, R.A. and Shikari, A.B., Efficient Callus Induction and Regeneration in Brassica juncea for Environment Friendly Agriculture, Int. J. Pure App. Biosci.5(1): 135-141 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2449
