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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (165) Last page : (172)
Article doi: http://dx.doi.org/10.18782/2320-7051.2864

Bio-efficacy of Steinernema carpocapsae against Spodoptera litura under Laboratory Condition

Saroj Yadav*, Jaydeep Patil and H.K. Sharma
Department of Nematology, Rajasthan College of Agriculture,
Maharana Pratap University of Agriculture and Technology, Udaipur-31300, India
*Corresponding Author E-mail: sarojhau29@gmail.com
Received: 18.04.2017  |  Revised: 29.04.2017   |  Accepted: 30.04.2017  


Tobacco caterpillar; Spodoptera litura (Fab.) (Lepidoptera: Noctuidae), has been recognised as most devastating pest of several cultivated crops, including vegetables throughout the world. Several bio-pesticides have been tested for the management of tobacco caterpillar in different parts of the country, but only few have shown promising results. Among different bio-agents, entomopathogenic nematodes were found comparatively better over others in suppressing the population of tobacco caterpillar. Laboratory studies was carried out to evaluate the efficacy of an indigenous population of Steinernema carpocapsae against fourth instar larvae of tobacco caterpillar. Mass multiplication of S. carpocapsae on different artificial media i.e., Nutrient broth, Wheat flour, Maize flour, Lipid and Modified Wout’s media. Different population of S. carpocapsae recovered from different artificial media of different dosage i.e., 100, 200, 300 and 400 IJs/Petri plate tested against S. litura. The per cent mortality of S. litura larvae after 96 hours was recorded, maximum 100 per cent mortality of S. litura was recorded at an inoculum level of 400 IJs/Petri plate recovered from Nutrient broth media. While, minimum 55 per cent mortality of S. litura was observed after 96 hrs at 100 IJs of S. carpocapsae produced on Wheat flour and Maize flour media respectively.

Key words: Entomopathogenic nematode, Steinernema carpocapsae, In vitro, Bio-efficacy, Inoculum level, Infective juveniles, Spodoptera litura.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Yadav, S., Patil, J. and Sharma, H.K., Bio-efficacy of Steinernema carpocapsae against Spodoptera litura under Laboratory Condition, Int. J. Pure App. Biosci. 5(2): 165-172 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2864