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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 2
First page : (740) Last page : (750)
Article doi: http://dx.doi.org/10.18782/2320-7051.2523

Evaluation of Hirsutum Cotton Genotypes for Water Stress using Peg-6000 by Slanting Glass Plate Technique

Megha B. R.1*, U. V. Mummigatti2, V.P. Chimmad3 and Y. R. Aladakatti4
1,3Dept. of Crop Physiology, UAS, Dharwad, Karnataka (580005), India
2,4Agriculture Research Station, Hebballi, UAS, Dharwad, Karnataka (582208), India
*Corresponding Author E-mail: megharangappa@gmail.com
Received: 27.01.2017  |  Revised: 8.02.2017   |  Accepted: 10.02.2017  


Laboratory experiment was conducted during 2016 at Agriculture Research Station, Crop physiology division, Dharwad, to screen cotton varieties to water stress condition for seed germination and seedling growth. Study consisted of 19 Gossypium hirsutum varieties with two checks. The genotypes were subjected to different osmotic potentials (0.0 MPa (0 bar), - 0.140 MPa (-1 bar) and -0.39 MPa (-3.9 bar)) by slanting glass plate technique. The genotypes were screened for percent seed germination, root length, shoot length and seedling vigour traits (seedling vigor index, shoot vigor index, and root vigor index) at 3rd, 7th and 12th day. Where, the germination percentage at different PEG-6000 concentrations is 87.6 %, 76.4 % and 19.0% and maximum germination per cent at higher PEG concentrations (20%) was 60% and at this concentration the shoot growth was completely inhibited in all the genotypes. As the PEG concentration increases there was a decrease in germination and increase in root to shoot ratio. The genotypes which found tolerant to the increased osmotic potential were Sahana, BS-37, LRA-5166, CCH-12-3 GBHV- 177, BS-39, GBHV-182, ARBH-1352, and the gneotypes RAH-806, TSH-4/115, CNH-1110, NDLH-1943, NDLH-1938, RAH-100 are less tolerant to different osmotic stress condition.

Key words: Osmotic potential, Hirsutum cotton, PEG-6000, Water stress

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Megha, B.R., Mummigatti, U.V., Chimmad, V.P. and Aladakatti, Y.R., Evaluation of Hirsutum Cotton Genotypes for Water Stress using Peg-6000 by Slanting Glass Plate Technique, Int. J. Pure App. Biosci.5(2): 740-750 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2523