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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (1) Last page : (9)
Article doi: http://dx.doi.org/10.18782/2320-7051.2953

Induced Systemic Resistance in Oilseed Rape by Some Bio-Elicitors Agents Against Rot Roots Diseases Caused by Rhizoctonia solani

Jawadayn Talib Alkooranee1*, Nada Nizar Kadhum2 and Tamarah Raad Aledan1 and Ilham Mohamed Hameed Al-farhan3
1University of Wasit, College of Agriculture, Department of Crops field, Wasit, Iraq
2Almssra School, Department of Alkarh /2 education, Education ministry, Baghdad, Iraq
3University of Misan, College of Agriculture, Department of Plant Protection, Misan, Iraq
*Corresponding Author E-mail: ja_alja@yahoo.com
Received: 6.05.2017  |  Revised: 16.05.2017   |  Accepted: 18.05.2017  


Rot roots disease in oilseed rape crop caused by phytopathogen Rhizoctonia solani. Four different bio-elicitors, Pseudomonas fluorescens PF83, Bacillus subtilis BS87, Trichoderma harzianum TH12 and T. viride TH10 tested to inhibition of R. solani, which reached 100%, 94.20%, 100% and 84.44% respectively. Their culture filtrates (CF) also were tested for growth inhibition of the pathogen in potato dextrose agar (PDA), which reached 66.07%, 55.72%, 63.90% and 54.00% respectively.
The degree of infection and disease severity of S. sclerotiorum were tested for their efficacy in oilseed rape Brassica napus in greenhouse conditions. Bio-elicitors and their CF demonstrated the ability to cause induced systemic resistance (ISR) in oilseed rape against rot root disease. Furthermore, a high ability to reduce the degree of infection in B. napus with the biotic elicitors P. fluorescens, B. subtilis, T. hazanim and T. viride was observed, with numbers reaching 5.67%, 10.33%, 7.00% and 14.67%, respectively. When CF was used, reached 15.00%, 25.33%, 19.67% and 33.00%, respectively, while for disease severity, with numbers reaching 4.33%, 7.00%, 6.67% and 10.33%, respectively. When CF was used, reached 12.33%, 20.00%, 17.67% and 28.33%.
These results show that biotic elicitor treatments can increase the fresh and dry weights of both roots and shoots as well as plant height compared with controls. Results suggest that bio-elicitors and their CF tested in this study can be used to control rot roots diseases of oilseed rape crop.

Key words: Oilseed rape, Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum and Trichoderma viride.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Alkooranee, J.T., Kadhum, N.N., Aledan, T.R. and Al-farhan, I.M.H., Induced Systemic Resistance in Oilseed Rape by Some Bio-Elicitors Agents Against Rot Roots Diseases Caused by Rhizoctonia solani, Int. J. Pure App. Biosci.5(3): 1-9 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2953