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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (989) Last page : (995)
Article doi: http://dx.doi.org/10.18782/2320-7051.2914

Genetic Analysis of Cyprinid Species from Kashmir Valley Based on Random Amplified Polymorphic DNA

Asifa Wali1*, Syed Mudasir Ahmad2, Imran Ganai3 and Masood-ul Hassan Balkhi1
1Faculty of Fisheries Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Rangil, Ganderbal Srinagar -19006, India
2Division of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama. Sher-e- Kashmir University of Agricultural Sciences and Technology, Kashmir, Shuhama Srinagar -19006, India
3Division of Animal nutrition, Faculty of Veterinary Sciences and Animal Husbandry, R.S.Pura.
Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, India
*Corresponding Author E-mail: asifawali@gmail.com
Received: 26.04.2017  |  Revised: 8.05.2017   |  Accepted: 10.05.2017  

 ABSTRACT

The  random  amplified  polymorphic  DNA-polymerase  chain  reaction  (RAPD-PCR)  was applied  to  analyze  the  genetic  variation  of  the  3  populations  of  cyprinid  species  Cyprinus carpio  var.  communis,  Cyprinus  carpio  var.  specularis   and  Carassius  carassius  collected from  different  locations  of  Kashmir,  India.  Twenty  five  samples  for  each  of  the  three species  collected  from  different  locations  were  used  for  this  study.  Of  the  12  random primers  used  8  generated  the  polymorphism  and  showed  82.23%  polymorphism.  The  study  showed  RAPD  marker  efficiently  helped  in  inter-  and  intra-  species  variation  of  the  three  species.  The  unweighted  pair  group  method  with  averages  (UPGMA) was  used to  construct  dendrogram  for  RAPD  which  resulted  in  two  clusters,  one  containing  C.  C.  Communis  and  C.  C.  specularis  and  the  other  included  the  C. carassius  species.  The Phylogenetic  analysis  demonstrated  that  scale  carp  (Cyprinus carpio communis)  is  closest  to  mirror  carp  (Cyprinus carpio specularis)  than  from  crucian  carp  (Crassius carassius).
Keywords:  Cyprinid, Genetic variation, RAPD, Polymorphism.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Wali, A., Ahmad, S.M., Ganai, I. and Balkhi, M.H., Genetic Analysis of Cyprinid Species from Kashmir Valley Based on Random Amplified Polymorphic DNA, Int. J. Pure App. Biosci.5(3): 989-995 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2914