International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (1025) Last page : (1034)
Article doi: http://dx.doi.org/10.18782/2320-7051.2633

Influence of Calpain Mediated Post-Mortem Ageing On Quality of Broiler Breeder Breast Fillets during Holding at Room Temperature

A. K. Giri, A. K. Biswas and O. P. Dinani*
Division of Post-Harvest Technology,
ICAR-Central Avian Research Institute, IVRI Canpus, Izatnagar, Bareilly-243122 (U.P.)
*Corresponding Author E-mail: dr_dinani@rediffmail.com
Received: 25.02.2017  |  Revised: 6.03.2017   |  Accepted: 7.03.2017  

 ABSTRACT

The aim of this study was to determine the activity of different calpains (µ and m) and calpastatin in broiler breeder breast fillets to understand their influence on ageing during holding at (27±2) °C. Both of the enzymes were extracted and they were subjected to casein zymography analysis. The purified enzymes were separated using anion exchange column chromatography, and their presence was confirmed by SDS-PAGE analysis. Casein Zymography results revealed the presence of µ-and m-calpains activity in breast fillets sample extracts. Though it has been observed that the band intensity keep on decreasing with the increasing ageing time which indicate the decrease in activity of these enzymes. The pH and Warner-Brazler Shear Force (WBSF) values were also decreased with the increase in ageing time while Lovibond tintometer colour, TBARS values, FFA contents and peroxide values were not much affected. Thus, it was concluded that both these enzymes are present in the muscle sample but that were autolyzed with the increase of aging. On the basis of results obtained it might be concluded that the optimum ageing time for broiler breeder breast fillets was optimized 4h at room temperature holding at(27±2) °C.

Key words: Calpains, Calpastatin, Casein zymography, Breast fillets

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Giri, A.K., Biswas, A.K. and Dinani, O.P., Influence of Calpain Mediated Post-Mortem Ageing On Quality of Broiler Breeder Breast Fillets during Holding at Room Temperature, Int. J. Pure App. Biosci.5(3): 1025-1034 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2633