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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 3
First page : (278) Last page : (283)
Article doi: http://dx.doi.org/10.18782/2320-7051.2881

Isolation and Characterization of Phosphate Sollubilizing Bacillus Consortia for Plant Growth Promotion

Krunal Modi1* and Feba Jacob2
1ASPEE SHAKILAM Agri. Biotechnology, Institute, Navsari Agricultural University, Surat, Gujarat, India
2College of agriculture, Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding Author E-mail: kgmodi@nau.in
Received: 21.04.2017  |  Revised: 30.04.2017   |  Accepted: 2.05.2017  


Twenty five rhizospheric soil samples were collected from five different sampling sites and a total of 29 phosphate sollubilizing Bacillus isolates were obtained. From primary and secondary screening of these isolates, best 5 phosphate sollubilzers SST3, SST4, SLYV3, SDR1, SDR2 were found to produce 15 µg/ml, 11 µg/ml, 13 µg/ml, 18 µg/ml and 12 µg/ml solubilised phosphate respectively. These isolates were further selected for preparing compatible consortia for on field application. Along with phosphate sollubilization, isolates SST3, SST4, SLYV3, SDR1 and SDR2 showed considerable PGPR activities like 54 µg/ml IAA, 50 µg/ml, 40 µg/ml IAA, 36 µg/ml IAA and 34 µg/mlIAA production; 1.2 µg/ml, 0.79 µg/ml, 0.83 µg/ml, 1.01 µg/ml and 1.35µg/mlprotease production. All the five isolates were found positive for  siderophore production, N2 fixation, cellulase production, HCN production while  isolate SST3 showed antifungal activity against fungal pathogen Colletotrichum falcatum. From 16S rRNA sequencing SST3, SST4, SLYV3, SDR1 and SDR2 isolates were identified as Bacillus cereus, Bacillus pumilus, Bacillus cereus Bacillus subterraneus, Bacillus cereus respectively.

Key words: Bacillus, Phosphate sollubilization, Plant Growth Promoting Rhizobactera, Bio fertilizer

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Modi, K. and Jacob, F., Isolation and Characterization of Phosphate Sollubilizing Bacillus Consortia for Plant Growth Promotion, Int. J. Pure App. Biosci.5(3): 278-283 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2881