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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (117) Last page : (122)
Article doi: http://dx.doi.org/10.18782/2320-7051.3097

Evaluation of Persistance and Efficiency of Different Biofilmed Biofertilizers with Chickpea Crop

Nissipaul. M.*, Sodimalla Triveni, R. Subhash Reddy and R. Sushma Devi
Department of Agriculture Microbiology & Bioenergy
College of Agriculture, PJTSAU, Ragendranagar, Hyderabad
*Corresponding Author E-mail: johnnissipaul@gmail.com
Received: 7.06.2017  |  Revised: 15.06.2017   |  Accepted: 18.06.2017  


Soil is considered to be a storehouse of microbial activity, though the space occupied by living organisms is estimated to be less than 5% of the total space. Soil samples were collected from the waste lands of Parthenium rhizosphere soils in college farm, Rajendranagar, Hyderabad and isolated different Rhizobacteria namely Bacillus subtilis, Pseudomonas flourescens and Trichoderma viride isolate from soil by serial dilution and plate count method. Rhizobium leguminosarum strain is collected from Biofertiliser unit, Agriculture Research Institute (ARI) Rajendranagar for the preparation of a Biofilm. A Biofilm is an aggregate of microorganisms in which cells are stuck to each other and/or to a surface. Plant-associated microorganisms fulfill important functions for plant growth and health. Such beneficial biofilms can be developed in vitro and be used as biofertilizers (Biofilmed Biofertilizers, BBs) and biocontroling agents for different crops, when applied at high cell densities. The research studies conducted so far in this field with special attention into development of biofilms of N2-fixing, P-solubilizing, and disease control bacteria and fungi. Four types of biofilms namely B. subtilis –T. viride, P. flourescense –T.viride, R. leguminosarum –T.viride B. subtilis –P. flourescense – R. leguminosarum –T. viride biofilms were prepared. Biofilm formation is a dynamic process involving quorum sensing for successful attachment and colonization. Regarding the persistence studies of biofilms in soil; the four types of biofilms were incubated in soil. Initial microbial populations were recorded. After 90 days of incubation, there is a decline in the counts of biofilm partners ranging from 50-73% under pot cultures were observed and recorded.

Key words: Bacillus subtilis, Pseudomonas flourescens, Rhizobium leguminosarum, Trichoderma viride.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Nissipaul, M., Triveni, S., Reddy, R.S. and Sushma, R.D., Evaluation of Persistance and Efficiency of Different Biofilmed Biofertilizers with Chickpea Crop, Int. J. Pure App. Biosci.5(4): 117-122 (2017). doi: http://dx.doi.org/10.18782/2320-7051.3097