International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (1980) Last page : (1988)
Article doi: http://dx.doi.org/10.18782/2320-7051.5293
Effect of Organic Solvents on the Activity and Stability of an Extracellular Protease Secreted by the Haloalkaliphilic Bacillus firmus HS4
Vijayshree M. Hemke1 and Vishal R. Dhundale2*
1Jijamata Mahavidyalay Buldhana, Maharashtra, India
2New Arts Commerce and Science College Ahmednagar, Maharashtra, India
*Corresponding Author E-mail: vrdvishal@gmail.com
Received: 23.07.2017 | Revised: 4.08.2017 | Accepted: 5.08.2017
ABSTRACT
Extremozymes are now-a-day replacing chemical catalyst in manufacturing of chemicals, textiles, pharmaceuticals, paper, food, leather processing and agricultural chemicals since the enzymes prepared with suitable properties with the advent of new knowledge in biotechnology.Partial characterization of protease from Bacillus was carried out by effect of pH, temperature, substrate, enzyme, metal ions and organic solvents on alkaline protease activity was then measured as per assay procedure. Total one hundred and fourteen isolates were obtained in the isolation exercise, cultural, morphological characteristics of all the strains were studied and fifty five isolates were selected on the basis of their pH salt and temperature tolerance bacteria were isolated from hypersaline alkaline lake. Out of them, a bacterium HS4 with an ability to produce extracellular halophilic, alkali-tolerant, organic solvent stable, and moderately thermostable protease producing strain was detected. Identification of the bacterium HS4 was done based upon biochemical tests and 16S rDNA sequence. The result of gene sequence analysis indicated that bacterium strain HS4 shows highest degree of similarities with Bacillus firmus. The optimum activity were revealed at 80°C, pH 10.5. Enzyme activity strongly exhibited enzyme activity by heavy metals such as BaCl2, CaCl2, MgCl2, FeCl3. Protease was found to be stable and enhance by organic solvents such as Methanol, Acetone, Benzene, ethanol, Hexane, propanol, ethyl acetate, xylene, acetic acid and Chloroform which has showed demonstrating the unique properties of protease enzymes. The enzyme is a haloalkaline, thermostable, metal ion and organic solvent tolerant in nature. The determination in the present study was an enzyme production analysis conducted to address this Bacillus firmus HS4 as a potential for biotechnology application.
Key words: Bacillus, Protease, Organic solvents, Metal ions.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Hemke, V.M. and Dhundale, V.R., Effect of Organic Solvents on the Activity and Stability of an Extracellular Protease Secreted by the Haloalkaliphilic Bacillus firmus HS4, Int. J. Pure App. Biosci.5(4): 1980-1988 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5293
