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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (22) Last page : (30)
Article doi: http://dx.doi.org/10.18782/2320-7051.5344

Total Polyphenols and Gallic Acid Contents in Domesticated Carob (Ceratonia siliqua L.) Pods and Leaves

El Bouzdoudi Brahim1*, Ammouri Najlae1, Joly Nicolas2, Martin Patrick2, Saïdi Rabah3, Nejjar El Ansari Zineb1, Bouras Mounia1, Badoc Alain4 and Lamarti Ahmed1
1Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco
2Département de Chimie, IUT Béthune, Université d’Artois, F-62408 Béthune, France
3Department of Matter and Life Sciences, High Normal School, BP 202, 93150 Martil, Morocco
4Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Œnologie EA 4577, USC 1366 INRA, UFR des Sciences Pharmaceutiques, Université de Bordeaux, ISVV (Institut des Sciences de la Vigne et du Vin), 210 Chemin de Leysotte, CS 50008, 33882 Villenave-d’Ornon, France
*Corresponding Author E-mail: bchiri2008@gmail.com
Received: 28.07.2017  |  Revised: 10.08.2017   |  Accepted: 12.08.2017  


Extraction condition efficiency of total polyphenols was studied on carob pods (Ceratonia siliqua L.) by performing extractions with various solvent systems, in order to evaluate and optimize the conditions of the recovery of polyphenols. Maximum amounts of polyphenols were found in 80% acetone extracts versus water inefficiency. Total polyphenol contents, determined by Folin-Ciocalteu method, were estimated at 11.19 mg/g (dry weight) and the chromatographic analyses demonstrated that gallic acid is the major polyphenol compound of the extracts (45% of polyphenols by dry weight material).
Carob leaves and different parts of fruits were also analyzed for their total polyphenols and gallic acid content. It was found that the mature leaves of the year contain more polyphenols and gallic acid (45.26 mg/g of total polyphenols and 17.01 mg/g of gallic acid). Tegument extracts contained lesser amounts of polyphenols and gallic acid (26.30 mg/g of total polyphenols and 2.51 mg/g of gallic acid), while only traces were detected in germ and endosperm (1.33 and 0.80 mg/g of total polyphenols; 0.99 and 0.72 mg/g of gallic acid, respectively). It should be noted that there are some additional phenolic compounds present whose structures should be precised.

Key words: Carob tree, Ceratonia siliqua, Extraction, Total polyphenols, Gallic acid.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: El Bouzdoudi, B., Ammouri, N., Joly, N., Martin, P., Saïdi, R., Nejjar El Ansari, Z., Bouras, M., Badoc, A. and Lamarti, A., Total Polyphenols and Gallic Acid Contents in Domesticated Carob (Ceratonia siliqua L.) Pods and Leaves, Int. J. Pure App. Biosci.5(4): 22-30 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5344