International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (2158) Last page : (2164)
Article doi: http://dx.doi.org/10.18782/2320-7051.5655
Evaluate the Shelf Life of Irradiated Carrier Based Pseudomonas Biofertilizer Stored At Different Temperatures at Different Intervals
G. Thirumal1*, R. Subhash Reddy2, S. Triveni3 and M.H.V. Bhave4
1,2,3Department of Agricultural Microbiology and Bioenergy
4Department of Statistics & Mathematics
College of Agriculture, Prof Jaya Shankar Telangana State Agricultural University,
Rajendranagar, Hyderabad - 500 030, Telangana, India
*Corresponding Author E-mail: thirumalgummula18@gmail.com
Received: 7.07.2017 | Revised: 28.07.2017 | Accepted: 30.07.2017
ABSTRACT
In this study focus on shelf life of different carrier based bioinoculants sterilized by irradiation, for this Pseudomonas cultures collected from different resource labs, then cultures were studied morphologically and biochemically for purity confirmation. The selected Pseudomonas (RGP-1) isolate was multiplied in large quantities in appropriate culture broth by incubating at 28 ± 2°C in an incubator shaker till they attained log phase with a cell load of l × l09cfu ml-1. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium Alginate carriers were selected and carriers sterilized by gamma irradiation at a dose rate of 5.0 kGy for 1h then carriers were used for bioinoculant preparation and stored at different temperatures i.e. 4ºC and 28± 2°C. In first month log10 values at 4ºC 9.65 (Vermicompost), 9.76 (Sodium Alginate), 9.64 ( Lignite), 9.67 (Vermiculate) and 28± 2°C 9.85 (Vermicompost), 9.92 (Sodium Alginate), 9.80 ( Lignite), 9.90 (Vermiculate) showed. Even though bioinoculant populations were higher at first month gradually deceasing upto 8th month, at the end ( 8th month) of the experiment log10 values were at 4ºC 8.40 (Vermicompost), 9.05 (Sodium Alginate), 8.00 ( Lignite), 8.70 (Vermiculate) showed which is highest value then minimum 5×107cfu g-1(7.6 log10) viable count of powdered form of carrier based biofertilizer and 28± 2°C was different viable count obtained 7.80 (Lignite) showed upto 6th month, 7.60 (Vermicompost), 8.16 (Sodium Alginate), 8.00 (Vermiculate) showed upto 7th month only.
Key words: Pseudomonas, Irradiated sterilization, Different temperatures, Sodium alginate.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Thirumal, G., Reddy, R.S., Triveni, S. and Bhave, M.H.V., Evaluate the shelf life of irradiated carrier based pseudomonas biofertilizer stored at different temperatures at different intervals, Int. J. Pure App. Biosci.5(4): 2158-2164 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5655
