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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 4
First page : (452) Last page : (461)
Article doi: http://dx.doi.org/10.18782/2320-7051.2436

Characterization of Xanthomonas oryzae pv. oryzae Isolates from Rice Growing Regions of Southern India

Shankara, K.1*, Patil, M. B.1, Pramesh, D.2, Gururaj Sunkad1, Yenjerappa, S. T.3, Ibrahim, M.3, Rajesh N. L.4 and Chikkannaswamy1
1Department of Plant Pathology, UAS Raichur, Karnataka, India
2Rice Pathology, ARS, Gangavathi, Karnataka, India
3ICAR-KVK, Hagari, Ballari, Karnataka, India
4Department of Soil Science and Agricultural Chemistry, UAS Raichur, Karnataka, India
*Corresponding Author E-mail: pathshankar.k@gmail.com
Received: 9.01.2017  |  Revised: 20.01.2017   |  Accepted: 21.01.2017  


Bacterial leaf blight (BLB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major biotic constraint in rice cultivation and is wide spread in Asia, including India. A total of 54 isolates were collected from different rice growing zones of southern India. All the isolates were obtained and subjected to pathogenicity test on BPT-5204 that varied significantly in terms of disease severity among each other and further confirmed with colony morphological features and biochemical tests. Colony size of the isolates ranged from 1.2 mm to 4.0 mm with light yellow to yellow and creamy yellow colour with circular to irregular margin. Most of the isolates produced raised, slimy colonies but few isolates produced flattened and slimy colonies. All the isolates were subjected to different biochemical tests and were found to be positive for 3 per cent KOH test, gelatin liquefaction, catalase test, while, negative for starch hydrolysis and oxidase tests. Five isolates namely, Xoo7, Xoo16, Xoo22, Xoo27 and Xoo50 found negative for H2S production test.

Key words: Bacterial leaf blight, Biochemical characterization, BLB, Rice, Morphology, Xanthomonas oryzae pv. oryzae

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Shankara, K., Patil, M.B., Pramesh, D., Sunkad, G., Yenjerappa, S. T., Ibrahim, M., Rajesh, N.L. and Chikkannaswamy, Characterization of Xanthomonas oryzae pv. oryzae Isolates from Rice Growing Regions of Southern India, Int. J. Pure App. Biosci.5(4): 452-461 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2436