International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (1286) Last page : (1297)
Article doi: http://dx.doi.org/10.18782/2320-7051.5470
Microbial Identification by Molecular Characterization and Screening of Phosphate Solubilizing Bacteria of Coriandrum sativum Rhizosphere
Aditi Tripathi1* and Farhat Banu2
1Research Scholar, Faculty of Science, Pacific Academy of Higher Education and Research University, Udaipur- 313024, Rajasthan, India
2Department of Botany, Govt. Meera Girls College, Udaipur, Rajasthan, India
*Corresponding Author E-mail: jvwu.aditi@gmail.com
Received: 19.08.2017 | Revised: 2.09.2017 | Accepted: 4.09.2017
ABSTRACT
The present study was conducted to analyze microbial diversity of Coriandrum sativum rhizosphere and isolate phosphate solubilising bacteria from soils of Hadoti, Rajasthan, India. In total fifty six bacteria were isolated from different soil samples collected from Kota, Bundi, Jhalawar and Baran districts of Hadoti region. These soil samples have been analyzed for their organic carbon, available nitrogen, electrical conductance (EC), pH, and temperature. Maximum EC 0.22 dS/m was recorded in Baran district and minimum EC 0.07 dS/m was recorded in Jhalawar district, while maximum pH was recorded 8.4 in Kota district of Rajasthan. Based on their size of halo zone formation on pikovaskaya medium, total ten bacterial isolates were selected as efficient phosphate solubilizing bacterial isolates. The Maximum phosphate solubilization in medium was observed in bacterial isolates COR-61. These isolates were also screened for IAA production, citrate utilization, catalase production, and to utilize different sources of carbohydrates and production of the specific enzymes. On the basis of morphological and biochemical characterization of the isolates we have identified them as members of the following species: Pseudomonas putida, Bacillus megaterium, Bacillus altitudinis, Micrococcus luteus, Bacillus subterraneus, Bacillus aryabhattai, Bacillus tequilensis, Pseudomonas sp. and Planococcus refietoensis. All the ten PSB (phosphate solubilizing bacteria) isolated from coriander seed spice soil of Hadoti region could efficiently solubilize tricalcium phosphate in the medium which could possibly help for future application in sustainable production of agriculture crops.
Key words: Coriander seed spice crop, Rhizosphere, Plant growth promoting rhizobacteria (PGPR), Phosphate solubilizing bacteria and biochemical and molecular analysis.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Tripathi, A. and Banu, F., Microbial Identification by Molecular Characterization and Screening of Phosphate Solubilizing Bacteria of Coriandrum sativum Rhizosphere, Int. J. Pure App. Biosci.5(5): 1286-1297 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5470
