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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (178) Last page : (182)
Article doi: http://dx.doi.org/10.18782/2320-7051.5453

Effect of Coumestrol Feeding on Sterilization of Male Dog

Rakesh Kumar1*, A. K. Sharma2 and Anand Kumar Pandey3
1Government Veterinary Hospital Kirmara, Hisar, Haryana, India
2Department of Veterinary Gynaecology and Obstetrics,
3Department of Veterinary Clinical Complex, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar (Haryana), INDIA
*Corresponding Author E-mail: dr.r.k.duggal@gmail.com
Received: 16.08.2017  |  Revised: 28.08.2017   |  Accepted: 1.09.2017  


The present study was conducted on ten healthy adult stray male dogs weighing 12-19 kg, and randomly divided into two groups.  Animals in treatment group were given coumestrol @ 1.5 mg/kg body weight (n=5) dissolved in di-methyl sulfoxide orally in a treat, once only. Dogs in control group (n=5) were given DMSO only. Castration of one dog was done on 12 hours, 24 hours and 7 days post feeding of coumestrol, respectively whereas two treated dogs were castrated on 15th day post feeding; same castration schedule was followed for control animals. Tissue from different parts of the testes were incised and fixed in Bouin’s fixative. Stages of seminiferous epithelial cycle and spermiogenesis were not identifiable in the treated dogs. However, no histopathological lesions were observed on structure of spermatogonial and sertoli cells. Therefore, it was concluded that oral feeding of coumestrol @ 1.5mg/kg b.w. cannot be used for sterilization of male dogs.

Key words: Coumestrol, Seminiferous epithelial cycle, Testes.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar, R., Sharma, A.K. and Pandey, A.K., Effect of Coumestrol Feeding on Sterilization of Male Dog, Int. J. Pure App. Biosci.5(5): 178-182 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5453