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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 5
First page : (15) Last page : (19)
Article doi: http://dx.doi.org/10.18782/2320-7051.2628

Seed Germination and Seedling Vigor Index in Bixa orellana and Clitoria ternatea

Manish Das1*, Manisha Sharma2 and Pramod Sivan3
2,3Senior Research Fellows,
1,2,3Directorate of Medicinal and Aromatic Plants Research, Anand – 387310, Gujarat
1Principal Scientist, Horticultural Science Division,
Krishi Anusandhan Bhawan-II, ICAR, New Delhi-110012, India
*Corresponding Author E-mail: manishdas50@gmail.com
Received: 19.04.2017  |  Revised: 25.05.2017   |  Accepted: 1.06.2017  


In the present investigation with seeds of two important medicinal plants viz. Bixa orellana L. (Annatto) and Clitoria ternatea L., optimal conditions for seed germination in Annatto were determined by subjecting the seeds to varied concentration of plant growth regulators earlier but hardseededness could not be broken. Thereafter, acid scarification (AS) with H2SO4 was done. Freshly harvested seeds (FH) and one year old (OYO) seeds were taken for the study. The study on effectivity of sulfuric acid at elevated moisture content (60 %) has been amply demonstrated in enhancing germination and reducing MGT in B. orellana. In C. ternatea, a 3×4×3 factorial experiment was set up to test the effects of moisture content with three levels of moisture of 12%, 10% and 5%, storage duration at 4 levels of 3, 6, 9 and 12 months and storage temperature at 3 levels of 30ºC, 20ºC and -20ºC on the seed germination.  All pretreatments significantly reduced the percentage of hard seeds as compared to non treated control seeds in Annatto. The percentage of hard seed decreased gradually as the pretreatment duration increased for all samples. The final germination percentage of all accessions, was significantly increased by the pretreatments. The total increase on average was 43.5 percent point for all samples (from 36.7 % of control to 80.2 % of pretreatments) in Annatto. The study suggests that the seeds of C. ternatea may possess physiological dormancy and seeds are moisture sensitive and therefore, altering moisture level is an effective method for breaking its physiological dormancy.

Key words: Bixa orellana, Clitoria ternatea, Seeds, Germination, Dormancy.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Das, M., Sharma, M. and Sivan, P., Seed Germination and Seedling Vigor Index in Bixa orellana and Clitoria ternatea, Int. J. Pure App. Biosci.5(5): 15-19 (2017). doi: http://dx.doi.org/10.18782/2320-7051.2628