International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1195) Last page : (1204)
Article doi: http://dx.doi.org/10.18782/2320-7051.5186
Effect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata
Ajeet Singh1*, Nitin Kumar Garg1, Mahesh Kumar Samota1, Sandeep Kumar Malyan2 and Shelly Praveen1
1Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110012, India
1CESCRA, Indian Agricultural Research Institute, New Delhi-110012, India
*Corresponding Author E-mail: dhakaajeet9961@gmail.com
Received: 10.07.2017 | Revised: 18.08.2017 | Accepted: 22.08.2017
ABSTRACT
Groundnut bud necrosis virus (GBNV) (Tospovirus genus, Bunyaviridae family) infects the major crops of solanaceae, leguminosae and cucurbitaceae in India. Temperature is an important factor which influences the plant growth and development under diseased conditions. Here, we investigated the effect of four temperatures (30°C, 25°C, 20°C and 15°C) on viral symptom expression and RNA accumulation in GBNV inoculated cowpea plants. The first visible symptom of GBNV infection at inoculated site was observed in the form of chlorotic spots which were converted into the necrotic spots as the infections succeed. Some yellow mosaic symptoms were also observed at the systemic site during viral infection cycle. Plants incubated at higher (30°C and 25°C) temperatures showed severe necrosis and higher viral RNA accumulation at inoculated site, and facilitated the viral spread at systemic site. However, viral RNA accumulation was less at systemic site than the inoculated site. In contrast, symptoms expression and viral RNA accumulation were decreased at inoculated site at low (20°C and 15°C) temperatures and no viral symptoms were observed at systemic site (15°C); viral RNA accumulation was also suppressed at this site. GBNV infection at the inoculated site induced the higher accumulation of H2O2 followed by the induction of cell death at higher temperatures (30°C and 25°C) than the lower (20°C and 15°C) temperatures. These results suggest that the accumulation of viral RNA accumulation parallel with the H2O2 production and induction of cell death by GBNV infection in cowpea plants is temperature dependent.
Key words: H2O2, Chlorosis, Necrosis, Hypersensitive response (HR), Programmed Cell Death (PCD).
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Singh, A., Garg, N.K., Samota, M.K., Malyan, S.K. and Praveen, S., Effect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata, Int. J. Pure App. Biosci.5(6): 1195-1204 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5186
