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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2017, Volume : 5, Issue : 6
First page : (1389) Last page : (1402)
Article doi: http://dx.doi.org/10.18782/2320-7051.5294

Designer Nucleases for Genome Editing in Plants: Different Approaches and Applications

Amardeep1*, Kisa Zaidi2, Shalu Sharma3 and Vikas Sharma4
1,4Chaudhary Charan Singh University, Meerut-250 004, Uttar Pradesh, India
2Department of Biosciences, Shri Ram College, Muzaffarnagar, Uttar Pradesh, India
3College of Applied Education & Health Sciences (CAEHS), Meerut-250 001, Uttar Pradesh, India
*Corresponding Author E-mail: amar92home@gmail.com
Received: 23.07.2017  |  Revised: 28.08.2017   |  Accepted: 4.09.2017  


Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Zinc-finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error prone nonhomologous end joining or homology-directed repair at specific genomic locations. In this review, recent approaches and progress of artificial nuclease technology in plants has been summarized and discussed.

Key words: ZFNs, TALENs, CRISPR/Cas, NHEJ, HDR.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Amardeep, Zaidi, K., Sharma, S. and Sharma, V., Designer Nucleases for Genome Editing in Plants: Different Approaches and Applications, Int. J. Pure App. Biosci.5(6): 1389-1402 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5294