International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (920) Last page : (926)
Article doi: http://dx.doi.org/10.18782/2320-7051.6101
In vitro Regeneration of Cultivars of Colocasia esculenta (L.) Schott
Nagaraj Anupama1, Defedar Hanumanthaiah Tejavathi2* and Varadahally Rangaiah Devaraj1
1Department of Biochemistry, Bangalore University, Central College, Bangalore- 560001
2Department of Botany, Bangalore University, Jnana Bharathi Campus, Bangalore-560056
*Corresponding Author E-mail: tejavathi_hanu@yahoo.com
Received: 15.12.2017 | Revised: 22.01.2018 | Accepted: 28.01.2018
ABSTRACT
Background and Aim: Colocasia esculenta, a member of Araceae is one of the major tuber crops grown widely throughout the Pacific islands, Asia and Africa. Corms are good source of starch and known for its medicinal value. Since traditional propagation is time consuming and labour intensive, in vitro techniques is the alternative strategy to overcome these limitations. Further, in vitro technology is well established as one of the viable method to conserve the elite germplasms. Hence, an attempt is made in the present investigation to multiply the three elite cultivars of Colocasia esculenta by employing in vitro techniques.
Materials and Methods: Standard tissue culture techniques were followed to raise the cultures. Segments of corms with buds of three elite cultivars- Muktakeshi, Telia and Sree Reshmi, procured from Central Tuber Crops Research Institute, India, were cultured on MS and L2 media supplemented with different concentrations and combinations of growth regulators. Cultures were maintained under controlled conditions of light and temperature.
Results: Both direct and indirect organogenesis were observed from the cultures. M S media supplemented with NAA (10.74µM) and BAP (17.76µM) was found to be more suitable for proliferation of shoot buds from explants without the formation of callus. Whereas, L2 fortified with 2, 4-D (9.04µM) and Kin (6.97µM) has induced profuse callus and subsequent regeneration of shoots. Thus, obtained shoots from both direct and indirect pathways were rooted on the same media. They were acclimatized in a potting mixture of sand and cocopeat in the ratio of 1:1. Nearly 94% of the regenerated shoots were survived after transferring them to field.
Key words: Colocasia esculenta, Cultivars, Callus, Micropropagation, Conservation.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Anupama, N., Tejavathi, D.H. and Devaraj, V.R., In vitro Regeneration of Cultivars of Colocasia esculenta (L.) Schott, Int. J. Pure App. Biosci.6(1): 920-926 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6101
