International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (933) Last page : (938)
Article doi: http://dx.doi.org/10.18782/2320-7051.5304
Analysis of Genetic Diversity in Potato (Solanum tuberosum L.) Genotypes through RAPD (Randomly Amplified Polymorphic DNA) Markers
Rajani* and Dhirendra Singh
Reseach Scholar, G. B. Pant University of Agriculture & Technology
*Corresponding Author E-mail: rawat.rajni08@gmail.com
Received: 26.07.2017 | Revised: 30.08.2017 | Accepted: 4.09.2017
ABSTRACT
Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular diversity of 50 potato genotypes in Tarai region of Uttarakhand. DNA was extracted from tender leaves of potato genotypes for PCR amplification. The PCR amplified DNA profile was visualized on 1.5% agarose gel, staining with ethidium bromide. Twenty RAPD primers were used and out of twenty only eight were amplified and gave polymorphic results. Therefore, eight RAPD primers were used to characterize the genetic diversity among the 50 potato genotypes. A total 84 reproducible loci were obtained with 8 RAPD primers. Each primer varied greatly in their ability to resolve variability among the genotypes. The primers amplified minimum 6 (OPH-16) to maximum 13 (OPA-18) loci with an average of 10.5 loci per primer (Table 1). Out of 84 bands, 78 were polymorphic and 6 were monomorphic (Table 3). The polymorphism percentage ranged between 81.81 (OPX-02) to 100 per cent (OPH-16, OPH-19, OPA-12 and OPA-18). Average polymorphic band was recorded to be 10 per primer.
Key words: Solanum tuberosum L., Genetic Diversity, Randomly Amplified Polymorphic DNA Cluster analysis, Dendrogram.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Rajani and Singh, D., Analysis of Genetic Diversity in Potato (Solanum tuberosum L.) Genotypes through RAPD (Randomly Amplified Polymorphic DNA) Markers, Int. J. Pure App. Biosci.6(1): 933-938 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5304
