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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1322) Last page : (1327)
Article doi: http://dx.doi.org/10.18782/2320-7051.5791

Quantification of Capsaicin and Ascorbic Acid Content in Twenty Four Indian Genotypes of Chilli (Capsicum annuum L.) by HPTLC and Volumetric Method

Kalyani Pradhan1*, Alok Nandi2, Anathbandhu Das3, Narayan Sahu4,  Niranjan Senapati2,
Siba Prasad Mishra5, Anjana Patnaik2 and Geeta Pandey 2
2All India Coordinated Research Project on Vegetable Crops,
3Department of Biotechnology, 4Central Laboratory,
1Ph.D Scholar, 5Department of Vegetable Science
Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
*Corresponding Author E-mail: kalyanipradhan1@gmail.com
Received: 25.09.2017  |  Revised: 30.10.2017   |  Accepted: 4.11.2017  


Capsaicin and ascorbic acid contents of twenty four Indian chilli varieties/accessions from Capsicum annuum (2011/CHIVAR-1,2011/CHIVAR-2, 2011/CHIVAR-3, 2011/CHIVAR-4, 2011/CHIVAR-5, 2011/CHIVAR-6, 2011/CHIVAR-7, 2011/CHIVAR-8, 2011/CHIVAR-9, KA-2(C), LCA-334(C), UTKAL AVA(L.C), 2012/CHIVAR-2, 2012/CHIVAR-3, 2012/CHIVAR-4, 2012/CHIVAR-5,  2012/CHIVAR-6,  2012/CHIVAR-8,  2012/CHIVAR-9, 2013/CHIVAR-1, 2013/CHIVAR-2,  2013/CHIVAR-3, 2013/CHIVAR-4, UTKAL RASHMI(L.C) were determined using High Performance Thin Layer Liquid Chromatography (HPTLC) and Volumetric method. Based on their pungency value, all the chilli accession/varieties were classified as highly pungent peppers. The accession 2011/CHIVAR-6 recorded maximum capsaicin content 50890mg/100g DW followed by 2011/CHIVAR-5 45800mg/100g DW, LCA-334 44540mg/100g DW and 2011/CHIVAR-7 40300mg/100g DW were observed to be similar and better than Utkal Ava 1070mg/100g DW KA-2 2890mg/100g DW, Utkal Rashmi 3430mg/100g DW. The minimum 500mg/100g DW was recorded by the genotype 2011/CHIVAR-8.  In case of green chilli, maximum ascorbic acid was recorded in 2011/CHIVAR-2 (112 mg 100 g-1) closely followed by 2012/CHIVAR-4 (101.33 mg 100 g-1), 2011/CHIVAR-6 (77.33 mg 100 g-1) and 2011/CHIVAR-9 (74.67 mg 100 g-1).  Lowest ascorbic acid content was found in 2013/CHIVAR-2 (26.67 mg 100 g-1). Similarly, Ascorbic acid content of red ripe fruits of the chilli genotypes, had a wide range of variation (45.33 mg to 317.33 mg 100 g-1 FW). Maximum ascorbic acid  content of 317.33 mg 100 g-1 FW was recorded in 2011/CHIVAR-3 followed by 2011/CHIVAR-4 (304 mg 100 g-1 FW) and 2011/CHIVAR-1 (290.67 mg 100 g-1) which were better than the rest. The genotype KA-2 recorded minimum ascorbic acid content of 45.33 mg 100g-1 FW. The variability in capsaicin and ascorbic acid content presented in the pepper germplasm can be exploited for breeding cultivars with improved nutritional qualities.

Key words: Capsicum annuum, Pungency, Vitamin C.  

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Pradhan, K., Nandi, A., Das, A., Sahu, N., Senapati, N., Mishra, S.P., Patnaik, A. and Pandey, G., Quantification of Capsaicin and Ascorbic Acid Content in Twenty Four Indian Genotypes of Chilli (Capsicum annuum L.) by HPTLC and Volumetric Method, Int. J. Pure App. Biosci.6(2): 1322-1327 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5791