Home >> Archives

International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1392) Last page : (1398)
Article doi: http://dx.doi.org/10.18782/2320-7051.6354

In-Silco Analysis of Fish Antifreeze Proteins and their Physicochemical Characterization

Mukesh Kumar1*, Hanzel Saldana2, Roshan Kumar Ram1,
Himanshu Bhattacharyya1, Norine D’ Souza2
1Fish Genetics and Biotechnology Division, ICAR- Central Institute of Fisheries Education,
Versova, Mumbai, Maharashtra 400061
2Department of Biotechnology, St. Xavier College, Mahapalika Marg, Mumbai, Maharashtra 400001
*Corresponding Author E-mail: mukesh.fbtpa503@cife.edu.in
Received: 5.01.2018  |  Revised: 13.02.2018   |  Accepted: 18.02.2018  


Antifreeze protein’s (AFP) are proteins which have an important function of ice binding and growth inhibition of ice crystals and found in plants, bacteria, fungi, invertebrates and fish.  In this present study 17 Sequence of fish antifreeze proteins were selected for their Physiochemical properties, Secondary structure, Homology modeling, validation, and for functional analysis. Insilco tools were used to describe the physiochemical, functional and structural properties of these protein. Physico-chemical properties like as molecular weight, amino acid composition, isoelectric point, charge residue, Grand average of hydropathicity, instability index are computed .The studies give insight into fish antifreeze protein structure.

Key words: Antifreeze protein, Fish, Isoelectric point, Ice binding, Physiochemical properties.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar, M., Saldana, H., Ram, R.K., Bhattacharyya, H. and D.’ Souza, N., In-Silco Analysis of Fish Antifreeze Proteins and Their Physicochemical Characterization, Int. J. Pure App. Biosci.6(1): 1392-1398 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6354