International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 1
First page : (1414) Last page : (1420)
Article doi: http://dx.doi.org/10.18782/2320-7051.5582
In vitro Response of Nine Different Genotypes of ‘Safed musli’ (Chlorophytum borivilianum) using Crown Shoot Bud as an Explant
Shikha Desai1, Parth Desai1*, Mounil Mankad1, Armi Patel1,
Ghanshyam Patil1 and Subhash Narayanan1
1&1* Plant Tissue Culture Laboratory, Anand Agricultural University, Anand- 388110, Gujarat.
*Corresponding Author E-mail: shikhabt19@gmail.com
Received: 1.09.2017 | Revised: 7.10.2017 | Accepted: 13.11.2017
ABSTRACT
Chlorophytum borivilianum, commonly known as ‘Safed musli’, is a historic medicinal herb. Explants were surface sterilized using sequential application of carbendazim-50%, streptocycline, kanamycin and 0.1% mercuric chloride. Sprouts were induced on Murashige and Skoog (MS) medium supplemented with 1 mgl-1 Benzylaminopurine (BAP) and 1 mgl-1 Kinetin. The shoot multiplication was carried out on MS supplemented with 2 mgl-1 BAP and 3 mgl-1 Kinetin. In vitro rooting was achieved on half strength MS medium supplemented with 2 mgl-1 Indole-3-butyric acid (IBA). Maximum number of shoots (12.75 ± 1.32) as well as roots (15.75 ± 1.26) was recorded for Vireshwar genotype while minimum number of shoots (3.63 ± 0.56) and roots (2.88 ± 0.40) was recorded for Pipalakhunt. Hardening of the in vitro rooted plants was achieved in sand, soil and cocopeat mixtures.
Key words: Chlorophytum borivilianum, Crown shoot bud, Nine genotypes.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Desai, S., Desai, P., Mankad, M., Patel, A., Patil, G. and Narayanan, S., In vitro Response of Nine Different Genotypes of ‘Safed musli’ (Chlorophytum borivilianum) using Crown Shoot Bud as an Explant, Int. J. Pure App. Biosci.6(1): 1414-1420 (2018). doi: http://dx.doi.org/10.18782/2320-7051.5582
