ISSN : 2320-7051

  • No. 772, Basant Vihar, Kota

    Rajasthan-324009 India

  • Call Us On

    +91 9784677044


International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (1081) Last page : (1084)
Article doi: :

Cellulase Production from Aspergillus niger using Paddy Straw as a Substrate and Immobilization

S. Ranjith, D. Kanchana and S. Pratheep
PG Agriculture Microbiology, Assistant Professor
Department of Agricultural Microbiology, Annamalai University, Chidambaram, India
*Corresponding Author E-mail:
Received: 15.03.2018  |  Revised: 22.04.2018   |  Accepted: 27.04.2018   



Conversion of lignocellulosic waste to sugars is important application of microbial cellulases. In objective of the present study is isolated the Aspergillus niger from spoiled coconut and produce cellulase enzyme using paddy straw as the substrate. The activity was evaluated using CMC method, the growth of the fungi was high at 100 mg/ml glucose concentration (0.65 at 540 nm) and Optimization of cellulase production was done by using various physical parameters (Temperature, pH). Cellulase production was maximum at the temperature 20°C and minimum at 40ºC. The optimal pH for the cellulase production was observed maximum in 5.5 and minimum in 7.0. Enzyme was immobilized using different size of alginate beads. The efficiency of enzyme high in 3.0 mm (90.93%) size of the beads.

Key words: Cellulase, Aspergillus niger, Immobilization, Lignocellulose.

Full Text : PDF; Journal doi :

Cite this article: Ranjith, S., Kanchana, D. and Pratheep, S., Cellulase Production from Aspergillus niger using Paddy Straw as a Substrate and Immobilization, Int. J. Pure App. Biosci. 6(2): 1081-1084 (2018). doi: