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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (1081) Last page : (1084)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6339

Cellulase Production from Aspergillus niger using Paddy Straw as a Substrate and Immobilization

S. Ranjith, D. Kanchana and S. Pratheep
PG Agriculture Microbiology, Assistant Professor
Department of Agricultural Microbiology, Annamalai University, Chidambaram, India
*Corresponding Author E-mail: s.ranjith994@gmail.com
Received: 15.03.2018  |  Revised: 22.04.2018   |  Accepted: 27.04.2018   

 

 ABSTRACT

Conversion of lignocellulosic waste to sugars is important application of microbial cellulases. In objective of the present study is isolated the Aspergillus niger from spoiled coconut and produce cellulase enzyme using paddy straw as the substrate. The activity was evaluated using CMC method, the growth of the fungi was high at 100 mg/ml glucose concentration (0.65 at 540 nm) and Optimization of cellulase production was done by using various physical parameters (Temperature, pH). Cellulase production was maximum at the temperature 20°C and minimum at 40ºC. The optimal pH for the cellulase production was observed maximum in 5.5 and minimum in 7.0. Enzyme was immobilized using different size of alginate beads. The efficiency of enzyme high in 3.0 mm (90.93%) size of the beads.

Key words: Cellulase, Aspergillus niger, Immobilization, Lignocellulose.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ranjith, S., Kanchana, D. and Pratheep, S., Cellulase Production from Aspergillus niger using Paddy Straw as a Substrate and Immobilization, Int. J. Pure App. Biosci. 6(2): 1081-1084 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6339