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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 2
First page : (1648) Last page : (1658)
Article doi: : http://dx.doi.org/10.18782/2320-7051.6473

Phloroglucinol ACTS2 As A Neuroprotective Agent in Rotenone Induced Parkinsonism in SH-SY5Y Cells

Archana Imayavaramban¹ and Vijayalakshmi Krishnamurthy²*
¹Research Scholar, ²Associate Professor,
Department of Biochemistry, Bharathi Women’s College, Chennai, India
*Corresponding Author E-mail: viji42research@yahoo.co.in
Received: 12.03.2018  |  Revised: 19.04.2018   |  Accepted: 24.04.2018  

 ABSTRACT

Parkinson’s disease (PD) is a progressive neurodegenerative disorders characterized by impaired motor functions. SH-SY5Y is a dopaminergic neuronal cell line which has been used as an invitro model for neurotoxicity experiments. Phloroglucinol, a compound from Ecklonia cava belongs to the Rutaceae family. The present study was aimed to evaluate the neuroprotective effect of phloroglucinol against rotenone (Neurotoxin) induced SH-SY5Y cells which is the model for Parkinsonism. Cell viability was assessed by MTT assay, ROS production by DCFH-DA, nuclear damage by DAPI and the activity of Catalase (CAT), superoxide dismutase (SOD), level of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS) were done by standard protocols. The present study revealed the neuroprotective effect of Phloroglucinol by increasing the cell viability while rotenone decreased the same. Rotenone exhibited increased ROS generation and nuclear damage whereas Phloroglucinol diminished the same. SH-SY5Y cells exhibited increased activities of SOD and CAT, and decreased the levels of lipid peroxides and increased the level of GSH, when treated with Phloroglucinol. These results suggest that the phloroglucinol suppressed cell death in rotenone induced SH-SY5Y cells and prevents the oxidative stress. Thus, phloroglucinol may serve as a potent therapeutic agent by virtue of its multiple pharmacological properties in the treatment of neurodegenerative disorders including PD.
Key words: Phloroglucinol, SH-SY5Y cells, Rotenone, GSH, Antioxidant potential.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Archana, I. and Vijayalakshmi, K., Phloroglucinol ACTS2 As A Neuroprotective Agent in Rotenone Induced Parkinsonism in SH-SY5Y Cells, Int. J. Pure App. Biosci.6(2): 1648-1658 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6473