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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (116) Last page : (122)
Article doi: : http://dx.doi.org/10.18782/2320-6821

Cell Protectants, Adjuvants, Surfactant and Preservative and their Role in Increasing the Shelf Life of Liquid Inoculant Formulations of
Pseudomonas fluorescens

B. J. Praveen Biradar¹* and G. P. Santhosh²

Department of Agricultural Microbiology, College of Agriculture, Bheemarayangudi,
University of Agricultural Sciences, Raichur, 584104 (Karnataka)
*Corresponding Author E-mail: sgp2222@rediffmail.com
Received: 5.07.2018  |  Revised: 13.08.2018   |  Accepted: 18.08.2018  

 

 ABSTRACT

The cell protectants viz., polyvinlypyrrolidone (PVP, 2%), polyethylene glycol (PEG, 1%), gum arabic (0.8%) and sodium alginate (0.1%), adjuvants like xanthan gum (0.3%) and carboxymethyl cellulose (CMC, 0.1%), Tween 20 (0.5%) as a surfactant and potassium sorbate (0.2%) as a preservative were used in preparation of liquid inoculant formulation of Pseudomonas fluorescens. These liquid inoculants were stored in BOD incubator at 28±2 °C for a period of 180 days. The inoculant containing cell protectant, polyvinlypyrrolidone (2%), adjuvant xanthan gum (0.3%), Tween 20 (0.5%) as surfactant and potassium sorbate (0.2%) as preservative retained 1.76 X 1010 CFU/ml at the end of 180 days of storage. These formulated liquid inoculants had both extended shelf life and viability.

Key words: Formulation, Liquid inoculant, Pseudomonas fluorescens, Shelf-life.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Praveen Biradar, B.J. and Santhosh, G.P., Cell Protectants, Adjuvants, Surfactant and Preservative and their Role in Increasing the Shelf Life of Liquid Inoculant Formulations of Pseudomonas fluorescens., Int. J. Pure App. Biosci.6(4): 116-122 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6821