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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2018, Volume : 6, Issue : 4
First page : (123) Last page : (133)
Article doi: :

Role of Polymeric Additives in Formulation, Shelf-life and Bioefficacy of Liquid Inoculant of Pseudomonas fluorescens

B. J. Praveen Biradar1* and G. P. Santhosh2

Department of Agricultural Microbiology, College of Agriculture, Bheemarayangudi,
University of Agricultural Sciences, Raichur, 584104 (Karnataka)
*Corresponding Author E-mail:
Received: 5.07.2018  |  Revised: 14.08.2018   |  Accepted: 20.08.2018  



In the present study liquid inoculant formulation of Pseudomonas fluorescens were developed with the use of polymeric additives to study the shelf-life and to evaluate the bioefficacy of Pseudomonas fluorescens. The polymeric additives were used in different combinations and the prepared liquid inoculant formulations of Pseudomonas fluorescens were assessed for their shelf-life at monthly intervals and were evaluated for their bioefficacy against the Fusarium (Fusarium oxysporum f.sp.lycopersici) wilt of Tomato under greenhouse conditions. Polymeric additives used were cell protectants viz., polyvinlypyrrolidone (PVP, 2%), polyethylene glycol (PEG, 1%), gum arabic (0.8%) and sodium alginate (0.1%); adjuvants viz., xanthan gum (0.3%) and carboxymethyl cellulose (CMC, 0.1%); surfactant used was Tween-20 (0.5%) and preservative was potassium sorbate (0.2%). LIF (Liquid inoculant formulation; T5) retained 1.76 X 1010 CFU/ml upto 180 days of storage. Even the tomato seedlings treated with the LIF T5 showed best results in all the growth and yield parameters studied including the disease incidence in these plants. The formulated liquid inoculants were found to have enhanced shelf-life and improved viability.                 

Key words: Liquid inoculant formulation, polymeric additives, shelf-life, Pseudomonas fluorescens

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Cite this article: Praveen Biradar, B.J., Santhosh, G.P., Role of Polymeric Additives in Formulation, Shelf-life and Bioefficacy of Liquid Inoculant of Pseudomonas fluorescens, Int. J. Pure App. Biosci.6(4): 123-133  (2018). doi: